A fasciclin-like arabinogalactan protein gene(FLA) was isolated from Brassica napus by homologous sequence cloning method,and designated as BnFLA.The open reading frame(ORF) of BnFLA is 1 200 bp in length,and the deduced protein encodes 399 amino acids with a calculated molecular weight of 42 885.9 Da and an isoelectric point of 6.37.Putative BnFLA protein harbours an N-terminal signal peptide,two AGP-like domains,two fasciclin-like domains and a GPI-anchor sequence in the C-terminus.Phylogenetic analysis indicated that amino acid sequences of BnFLA were closer to BrFLA17 and AtFLA2 than other FLAs,sharing 98% and 87% identity,respectively.qRT-PCR analysis showed BnFLA was expressed in all B.napus tissues analysed,with the highest expression level in hypocotyls,moderately expressing in cotyledons,and the lowest expression in stems.BnFLA expression is induced by GA₃,BR,IAA,ABA and NaCl,but inhibited by 6-BA,sucrose,cold and PEG.This study suggested that BnFLA might participate in the signal transduction pathways of hormones and response to abiotic stresses in B.napus.