Adventitious buds regenerated through Agrobacterium-mediated Jatropha curcas L.transformation systems are usually difficult to develop to whole plantlets.In order to solve the problem,we tested in vitro micrografting methods by using J.curcas buds and shoots isolated from seedlings as scions and the lower part of J.curcas seedlings as stocks.The experimental results showed that:(1)Antibiotics could hinder the further growth of grafted seedlings significantly;(2)The most suitable stocks were those prepared from 5-day-old seedlings;(3)The hypocotyls with the upper part of the radicals were the most appropriate type of stocks;(4)Significant enhancement on the growth of buds in culture was confirmed in 1/2 MS medium containing 0.3 mg/L indole-3-butyric acid (IBA) and 2 mg/L L-glutamine (Gln);(5)The grafted seedlings could be transplanted to soil with quite high survival rate (76.40%);(6) The grafted seedlings with shoots or buds served as scions could grow normally.The growth of the transformed buds and shoots could be enhanced effectively by making use of the established in vitro micrografting system.