Abstract:Kiwifruit is dioecious, Hardy kiwifruit (Actinidia arguta Planch.) cultivars ‘Longcheng 2’and ‘Huanyuo 1’ cultivated in DandongKuandian area can still normally bear fruit and the fruit can produce seeds in the absence of pollination male plant. In response to the phenomenon, this article attempts to definite the reproductive characteristics through the determination of pollen viability, observation of pollen morphology by scanning electron microscope, different pollination bag technique and observation of the pollen tube behavior by fluorescence microscopy, and provide the theories basis for hardy kiwifruit planting and production. The results were summarized as follows: (1) the pollens of ‘Longcheng 2’ and ‘Huanyuo 1’ had no vitality. (2) The pollen grains of ‘Longcheng 2’and ‘Huanyuo 1’ were hollow and infertile, there was a small amount of male pollen found on the carrying pollen leg of honeybee. (3) There was no pollen germination in selfpollination, and the pollen germination rate of artificial pollination (Lüwang) was significantly higher than that of natural pollination. (4)There was no fruit set in bagged flowers. The fruit setting rate,fruit weight, fruit length and diameter of artificial pollination were slightly higher than that of natural pollination, and fruit side diameter and number of seeds per fruit of the former were significantly higher than that of the latter.(5)The wild Actinidia can be found around in the mountain which was about 1 km away from the cultivated area. In conclusion:‘Longcheng 2’, and ‘Huanyuo 1’ are female cultivars,the reason of which can bear fruit without pollinator tree in DandongKuandian area was that there were wild A. arguta surrounding the planted aera, bees are the main pollinators,and fully pollination contributes to enhancing its fruit set and fruit size. Therefore, it is necessary to configure male plant in the normal cultivation.