建兰花色形成的分子调控机理研究
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河南省科技攻关(国际合作领域)项目(172102410053);


Molecular Regulation Mechansim of Floral Color Formation in Cymbidium ensifolium
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    摘要:

    为探究建兰花色形成的分子调控机理,该研究以同株建兰黄绿色、红色花瓣为实验材料,采用高通量Highseq测序技术进行转录组文库构建,从基因水平探索花色物质的合成代谢通路及关键调控基因的转录活性。结果表明:(1)转录组测序共获得131 110 030条过滤序列(Clean Reads)、106 479条单基因(Unigenes),通过NR、GO、COG、KEGG等公共数据库比对,获得了29 748个有注释信息的Unigenes。(2)建兰黄绿色花瓣与红色花瓣中包括20个类黄酮合成代谢相关差异表达基因,与黄绿色建兰花瓣相比,红色花瓣中776个基因转录表达量上升,589个基因转录表达量下降,在KEGG数据库被注释到93条代谢通路,涉及6条类黄酮合成代谢相关的途径,共20个差异表达基因(83 Unigenes)。(3)QRTPCR分析显示,所选20个基因在建兰2种颜色花瓣中的表达量比值趋势与转录组FPKM比值趋势一致,表明该研究获得的转录组数据具有较高的参考价值;其中分支酸、苯丙氨酸解氨酶、肉桂酸4羟化酶、4香豆酸:辅酶A连接酶基因表达上调,有利于类黄酮前体积累;查尔酮合成酶、二氢黄酮醇还原酶、花青素合成酶基因在黄绿色花瓣中几乎不表达,在红色花瓣中表达明显上调,可能与建兰花色形成相关。

    Abstract:

    In order to explore the molecular mechanism of the formation of Cymbidium ensifolium flowercolor, we used highthroughput highseq sequencing technique to construct transcriptome library with the yellowgreen and red petals of one Cymbidium plant as experimental materials in this study. We investigated about the biosynthesis metabolism pathway of color related ingredients, the transcriptional activity of key regulatory genes, etc. The results showed that: (1) a total of 131 110 030 Clean Reads and 106 479 Unigenes were obtained by transcriptome sequencing, and 29 748 Unigenes with annotated information was obtained by comparing with NR, GO, COG, KEGG and other public databases; (2) in contrast to yellowgreen petals, the expression of 776 genes increased and 589 genes decreased in red petals, were identified to 93 metabolic pathways by KEGG database, and of which 6 pathways and total 20 differentially expressed genes (83 Unigenes) are related to flavonoid biosynthesis; (3) qRTPCR analysis showed that the expression ratio trend of the selected 20 genes in Cymbidium petals is consistent with the transcriptome ratio trend of FPKM, which means the transcriptome data obtained in this study has a high reference value. Among them, the upregulation genes of chorismate mutase (CM), phenylalanine ammonialyase (PAL) and cinnamate 4hydroxylase (C4H), 4coumarate: CoA ligase (4CL) are in favor of flavonoid precursor accumulation. Chalcone synthase (CHS), dihydroflavonol4reductase (DFR) and anthocyanidin synthase (ANS) were almost no expressed in yellowgreen petals, but significantly increased in the red petals, which are possibly connected with the floral color formation of Cymbidium ensifolium.

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李文建,冯 景,贾文庆,等.建兰花色形成的分子调控机理研究[J].西北植物学报,2018,38(4):615-623

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  • 在线发布日期: 2018-05-21
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