Abstract:In this study, we used wild type of Arabidopsis thaliana as tested materials, and the CmSAMDC gene from melon was built into the pCAMBIA1304 binary vector, then the vector was transformed into A. thaliana by using Agrobacteriummediated method. The transgenic plants overexpressing CmSAMDC were obtained by screened on MS medium containing 50 mg/L hygromycin, and then the salt tolerance test was performed by T3 transgenic seedlings. The results showed that: (1) the plant overexpression vector 35S∷CmSAMDC was successfully constructed and finally the T3 transgenic A. thaliana plants were obtained after transformed by Agrobacterium and resistance screened by hygromycin. (2) The lateral roots of the transgenic T3 lines were stronger than that of wildtype under 100, 150 and 200 mmol/L NaCl treatment. Meanwhile, the transgenic T3 lines grew up normally under 200 mmol/L NaCl treatment by irrigating roots, while the wild type plants were significantly inhibited under the same condition. In addition, the T3 transgenic seedlings were still alive under 400 mmol/L NaCl treatment by irrigating roots 16 days later whereas the wildtype plants gradually died. Furthermore, the level of MDA was higher in wildtype plants than that of transgenic plants under the same salt stress. The results showed that overexpressing of CmSAMDC gene could obviously enhance the salt tolerance of transgenic A. thaliana.