We conducted pot culture experiment to study the effect of different nitrogen forms［NH₄⁺N, NO₃^-N, CO(NH₂)₂］ and ratios on the changes in nitrate and nitrite contents, the contents of nutrients and medicinal ingredients of Perilla frutescens (L.) Britt seedlings. We studied the impact of different nitrogen forms and ratios on the P. frutescens which mainly includes dynamic changes of nitrate and nitrite contents, nutritional components, mineral elements and secondary metabolites, in order to provides a theoretical basis for the rational application of nitrogen fertilizer in P. frutescens cultivation and production. The results showed that: (1) the contents of nitrate and nitrite in P. frutescens leaves accumulates with the time proceeding. Before harvesting, nitrate content reaches the lowest point when NH₄⁺N /NO₃^-N was 100∶0, the content of nitrite reaches the lowest point when NH₄⁺N/NO₃^-N was 25∶75. (2) The contents of soluble sugar and starch achieved the highest level when NH₄⁺N/NO₃^-N was 0∶100, but the contents of free amino acid and Vc achieved the highest level under amidenitrogen treatment; The contents of P, K and Ca had their maximum values when NH₄⁺N/NO₃^-N was 50∶50, the contents of Zn, Fe and Mn had their maximum values when NH₄⁺N/NO₃^-N was 100∶0, and the content of Mg was peaked when NH₄⁺N/NO₃^-N was 0∶100. (3) The contents of total flavonoids, volatile oil and rosmarinic acid firstly increased and then declined constantly along with the increase of NH₄⁺N/NO₃^-N ratio, and these contents peaked when NH₄⁺N/NO₃^-N was 25∶75; The relative content of anthocyanin achieved the highest level under amidenitrogen treatment. In the cultivation and production of P. frutescens, when the NH₄⁺N/NO₃^-N ratio of 25∶75, we can get the best medicinal quality and nutritional quality, and control the content of nitrite.