In this study, we used the new species Narcissus tazetta‘Yunxiang’ as the experimental material. A NAC gene was cloned by RTPCR and named NtNAC2， which contains 936 bp open reading frame encoding 311 amino acids. Bioinformatics analysis showed that NtNAC2 gene is a member of NAC family， which contains a typical NAM conserved domain located in the Nterminal region and a close evolutionary relationship grouped with monocotyledonous Asparagus officinalis， Crocus sativus， Dendrobium catenatum. The quantitative realtime PCR detection showed that the relative expression level of NtNAC2 gene in petal and corona increased gradually with the development of floral organs, and reached the peak in the senescence period. The expression profiles of NtNAC2 in root and leaf were analyzed， it was inducible under ABA， MeJA， SA， H₂O₂， 50 ℃， PEG and NaCl treatments， and the spatiotemporal and tissue expression difference of root and leaf was reflected．In order to further identify its abiotic stress function， we successfully constructed the plant overexpression vector and finally obtained 10 strains of transgenic tobacco by GUS and molecular detection. The overexpression of NtNAC2 in transgenic tobacco was detected by semiquantitative RTPCR. The roots of transgenic plants are 2 to 3 times that of wild type， the water loss rate of leaves is less than 30% and the survival rate is higher than 60% under the salt and drought stress. These results showed that the expression of NtNAC2 gene can increase the tolerance of tobacco to high salt and drought， so it can be used as an effective candidate gene for N. tazetta resistance molecular breeding.