茶树CsLEA5基因的克隆及表达分析
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中国博士后科学基金(2016M602873);


Cloning and Expression Analysis of CsLEA5 Gene in Tea Plant (Camellia sinensis)
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    摘要:

    该研究以茶树‘龙井长叶’为材料,克隆获得了茶树胚胎发育晚期丰富蛋白基因CsLEA5的cDNA序列,该序列全长515 bp,包含一个375 bp的开放阅读框,编码124个氨基酸,预测蛋白分子量为13.5 kD,理论等电点为5.92。蛋白序列分析结果显示,CsLEA5为高亲水性和稳定性蛋白,且含有一个典型的LEA_3保守结构域,属于LEA蛋白中LEA_3亚家族成员。CsLEA5基因启动子区域包含多种与逆境响应相关的顺式作用元件,如乙烯响应元件(ERE)、胁迫响应元件(STRE)、创伤响应元件(WUNmotif)及MYB、MYC转录因子识别位点等。qRTPCR分析显示,CsLEA5基因表达具有明显的组织特异性,在叶片中的表达量最高,其次是嫩茎,而在其他组织器官中的表达量较低,且CsLEA5基因表达受低温和干旱胁迫的诱导。研究表明,CsLEA5基因可能在茶树响应低温和干旱胁迫过程中发挥重要作用。该研究对了解茶树抗逆分子机制,筛选抗性候选基因资源提供了重要理论依据。

    Abstract:

    In this study, ‘Longjingchangye’ tea plant was used as tested material, and the cDNA sequence of the CsLEA5 gene in tea plant was cloned. The fulllength cDNA was 515 bp, including a 375 bp open reading frame that encodes 124 amino acids with a molecular weight of 13.5 kD and a theoretical pI of 5.92. Protein sequence analysis showed that CsLEA5 is a highly hydrophilic and stable protein, and contains a typical LEA_3 conserved domain, belonging to LEA_3 subfamily members of LEA protein. In addition, the promoter of CsLEA5 gene contains a variety of cisacting elements related to stress response, such as ethylene responsive element (ERE), stress responsive element (STRE), wound responsive element (wunmotif) and MYB, MYC transcription factor recognition sites, etc. The qRTPCR analysis showed that the expression of CsLEA5 gene had obvious tissue specificity, with the highest expression in leaves, followed by tender stem, and lower expression in other tissues and organs. Furthermore, the expression of CsLEA5 gene was induced by cold and drought stress, suggesting that it plays an important role in the response of tea plants to cold and drought stress. This study provides an important theoretical basis for understanding the resistance molecular mechanism of tea tree and screening the resistance candidate gene resources.

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任 燕,高 童,陈江飞,等.茶树CsLEA5基因的克隆及表达分析[J].西北植物学报,2018,38(12):2186-2193

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  • 在线发布日期: 2019-01-24
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