硬粒小麦 长穗偃麦草2E和4E附加系及E染色体传递
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引用本文:罗贤磊,戴 毅,陈士强,李海凤,段亚梅,陈建民.硬粒小麦 长穗偃麦草2E和4E附加系及E染色体传递[J].西北植物学报,2019,39(6):953~962
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罗贤磊1,戴 毅2,陈士强3,李海凤4,段亚梅1,陈建民1* (1 扬州大学 生物科学与技术学院江苏高校现代谷物生产协作中心江苏扬州 2250092 扬州大学 农业科技发展研究院农业与农产品安全国际合作联合实验室江苏扬州 2250093 江苏里下河地区农科所江苏扬州 2250074 扬州职业大学江苏扬州 225012) 
基金项目:江苏省自然科学基金(BK20180908,BK20181213);
中文摘要:为探讨长穗偃麦草E染色体在硬粒小麦背景中的传递特点,利用染色体特异分子标记、基因组原位杂交(GISH)、非变性荧光原位杂交(ND FISH)等方法,对小偃麦8801(AABBEE)与硬粒小麦(AABB)杂交后代中选育的株系Du_No.2和Du_No.4进行了分析。结果表明:(1)分子标记检测株系Du_No.2及Du_No.4分别能扩增出长穗偃麦草2E、4E染色体特异条带。(2)GISH和ND FISH分析显示,株系Du_No.2和Du_No.4分别附加了1条2E和4E染色体,表明株系Du_No.2 和Du_No.4分别为硬粒小麦 长穗偃麦草2E和4E单体附加系。(3)2个株系的减数分裂过程观察发现,后期Ⅰ、Ⅱ和末期Ⅱ都有E染色体分离异常现象,且株系Du_No.2和 Du_No.4的异常率分别为22.24%和36.18%。(4)2个株系分别与硬粒小麦进行正反杂交的后代PCR分析表明, 2E和4E染色体经雄配子的传递率分别为4.41%和2.17%,而通过雌配子的传递率都为零,表明2E和4E染色体在硬粒小麦背景中能通过雄配子传递,但不通过雌配子的传递。该研究为创建全套硬粒小麦 长穗偃麦草双体附加系及代换系提供基础。
中文关键词:硬粒小麦  长穗偃麦草  附加系  染色体传递
 
Durum Thinopyrum elongatum 2E and 4E Addition Lines and E Chromosome Transmission
Abstract:To explore the transmission characters of chromosomes from Thinopyrum elongatum in the background of durum, The lines Du_No.2 and Du_No.4 derived from a cross between Tritileytrigia 8801 (AABBEE) and T. turgidum L. ssp. durum (AABB) were characterized by chromosome specific molecular markers, genomic in situ hybridization (GISH), and non denaturing fluorescence in situ hybridization (ND FISH). The results showed that: (1) the molecular markers could amplify the specific bands of 2E and 4E chromosomes of Th. elongatum from line Du_No.2 and Du_No.4, respectively. (2) The analysis of GISH and ND FISH showed that the lines Du_No.2 and Du_No.4 were respectively added with a 2E and 4E chromosome, indicating that the line Du_No.2 and Du_No.4 were durum Th. elongatum 2E and 4E monosomic addition lines, respectively. (3) The meiosis process of two lines showed that both lines have E chromosome segregation abnormalities in anaphase Ⅰ, Ⅱ and telophase Ⅱ, and the abnormal rates of lines Du_No.2 and Du_No.4 were 22.24% and 36.18%, respectively. (4) PCR analysis of the progeny of the two lines with the durum showed that the transmission rates of 2E and 4E chromosomes through male gametes were 4.41% and 2.17%, respectively, while the transmission rates through female gametes were zero, indicating that 2E and 4E chromosomes could be transmitted by male gametes in the background of durum, but not through female gametes. This study provided a basis for the establishment of a complete set of durum Th. elongatum disomic addition lines and substitution lines.
keywords:durum  Thinopyrum elongatum  addition line  chromosome transmission
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