梅花2个SVP基因的克隆与表达分析
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引用本文:李玉舒,杨炜茹,程堂仁,王 佳,张启翔.梅花2个SVP基因的克隆与表达分析[J].西北植物学报,2019,39(7):1163~1171
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李玉舒1,2,杨炜茹1,程堂仁1,王 佳1,张启翔1* (1 北京林业大学 园林学院花卉种质创新与分子育种北京市重点实验室国家花卉工程技术研究中心城乡生态环境北京实验室北京 1000832 北京农业职业学院北京 102442) 
基金项目:国家“863”计划(2013AA102607);
中文摘要:SVP (SHORT VEGETATIVE PHASE)是MADS box家族一员,它能够整合多条开花途径的开花信号,调节植物由营养生长向生殖生长的转变。为了解梅花(Prunus mume Sieb. et Zucc.)成花转变过程的分子机理,该研究采用RT PCR方法从梅花‘长蕊绿萼’中克隆到2个SVP的同源基因,分别命名为PmSVP1和PmSVP2,并采用荧光定量PCR对2个基因的表达模式进行分析。序列分析表明,PmSVP1和PmSVP2的编码区长度分别为687 和672 bp,分别编码228和223氨基酸。系统进化结果显示,PmSVP1与拟南芥AtSVP以及一些木本植物SVP同源基因聚为一组,PmSVP2与马铃薯、乳浆大戟等草本植物中的SVP同源基因聚为一组。实时荧光定量分析表明,在成年梅花中,2个PmSVP基因主要在茎、叶和叶芽等营养器官中表达,且都在叶中表达量最高。在1月龄幼苗中,PmSVP1基因在根、茎、叶中都有表达,PmSVP2基因则没有任何表达;在梅花花芽分化过程中,PmSVP1和PmSVP2基因的表达量均呈现下调表达的趋势。研究推测,PmSVP1和PmSVP2基因可能参与调控梅花从营养生长向生殖生长的转变。
中文关键词:梅花  SVP  基因克隆  表达分析
 
Cloning and Expression Analysis of Two SVP Genes from Prunus mume
Abstract:The MADS box gene SVP (SHORT VEGETATIVE PHASE) integrates multiple flowering signals to regulate the transition from vegetative to reproductive development in plants. To better understand the molecular regulation of flower development in Prunus mume, we isolated two putative orthologs of SVPPmSVP1 and PmSVP2. The expression patterns of PmSVP1 and PmSVP2 were determined by real time RT PCR. PmSVP1 contains a 687 bp open reading frame (ORF) that encodes a peptide of 228 amino acid residues. PmSVP2 contains a 672 bp ORF that encodes a peptide of 223 amino acid residues. Phylogenetic analysis suggested that PmSVP1 was closely related to Arabidopsis SVP, along with SVP like members from some woody perennials, while PmSVP2 was closely related to EeDAM2 and StMADS11, which are SVP like members from perennial herbs. To characterize the function of PmSVP1 and PmSVP2, we examined the spatial expression of PmSVP1 and PmSVP2 in different organs using qRT PCR. Transcripts of PmSVP1 and PmSVP2 accumulated in vegetative organs, such as buds, stems and leaves. However, the expression level of PmSVP2 was expressed only in adults and had low expression levels in vegetative organs, thus differing from PmSVP1. Throughout the bud differentiation process, both PmSVP1 and PmSVP2 expression levels presented a downward trend. Once flower bud differentiation commenced, expression levels started to decrease, suggesting that these two genes may play an important role in the transition from the vegetative growth to the reproductive growth phase in P. mume.
keywords:Prunus mume  SVP gene cloning  expression analysis
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