葡萄风信子MaHY5基因的克隆与表达分析
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引用本文:曹诗梦,刘雅莉.葡萄风信子MaHY5基因的克隆与表达分析[J].西北植物学报,2019,39(7):1188~1194
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曹诗梦,刘雅莉* (西北农林科技大学 旱区作物逆境生物学国家重点实验室农业部西北地区园艺作物生物学与种质创制重点实验室陕西杨陵712100) 
基金项目:国家自然科学基金(31471905)
中文摘要:HY5(ELONGATED HYPOCOTYL5)是促进植物光形态建成过程中非常重要的转录因子。该研究以亚美尼亚葡萄风信子(Muscari armeniacum)为材料,克隆出1个bZIP型转录因子基因MaHY5(GenBank 登录号MK281355)。生物信息学分析显示,MaHY5基因开放阅读框为438 bp,编码145个氨基酸,蛋白质分子量约为16.1 kD,理论等电点为9.78,含有一个保守的bZIP特征结构域;MaHY5蛋白属于亲水性蛋白,无信号肽结构,属于非分泌蛋白,可能定位于细胞核。序列比对和进化树分析表明,HY5在进化过程中高度保守。实时荧光定量PCR和高效液相色谱检测分析表明,MaHY5基因的表达与花青素积累模式基本协同,都在花中优势表达,且在完全开放的花朵中表达较高。 推测MaHY5基因可能参与了葡萄风信子花色素积累过程。
中文关键词:葡萄风信子  HY5转录因子  基因克隆  花青素合成
 
Cloning and Expression Analysis of MaHY5 Transcription Factor from Grape Hyacinth
Abstract:HY5(ELONGATED HYPOCOTYL5) is an important transcription factor in photomorphogenesis process. We have cloned a bZIP transcription factor gene named MaHY5 from grape hyacinth(Muscari armeniacum) and its GenBank accession number is MK281355. Bioinformatics analysis of MaHY5 gene showed that it contains a 438 bp ORF that encodes a protein of 145 amino acid with an estimated molecular weight of 16.1 kD and an isoelectric point of 9.78. MaHY5 protein was not predicted to have an N terminal signal peptide or a transmembrane domain and it was predicated to be a hydrophilic protein that is localized to the nucleus. The protein contains an bZIP domain which is highly conserved in evolution process, analyzed by sequence alignment and phylogenetic analysis. The qRT PCR and HPLC result indicated that the expression of MaHY5 was general correlated with the mode of anthocyanin accumulation. They were both highly expressed in petals, especially in fully open flowers. Thus, MaHY5 may play a role in the process of anthocyanins accumulation in grape hyacinth.
keywords:grape hyacinth (Muscari armeniacum)  HY5 transcription factor  gene cloning  anthocyanin biosynthesis
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