续随子ElFAD2基因的序列及表达特性分析
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引用本文:任国鹏,葛丽萍,孙超超,李润植.续随子ElFAD2基因的序列及表达特性分析[J].西北植物学报,2019,39(7):1203~1211
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作者单位
任国鹏1,葛丽萍1*,孙超超1,李润植2 (1 山西农业大学 林学院山西太谷 0308012 山西农业大学 分子农业与生物能源研究所山西太谷 030801) 
基金项目:山西省林业科技创新项目(2018LYCX33);
中文摘要:为了研究油酸脱氢酶(FAD2)基因ElFAD2对续随子(Euphorbia lathyris L.)中不饱和脂肪酸合成的调控作用,该研究在续随子转录组数据的基础上经筛选获得ElFAD2基因序列,并对其序列及表达特性进行分析。序列分析结果显示,ElFAD2基因全长1 907 bp,ORF长1 152 bp,共编码383个氨基酸,包含有典型的脂肪酸去饱和酶结构域。续随子ElFAD2蛋白理论等电点为8.08,属于稳定蛋白,包含4个跨膜区和3个保守的组氨酸簇。基于FAD2的系统发育分析表明,续随子与同科植物乌桕(Triadica sebifera L.)的亲缘关系最近。荧光定量PCR分析发现,ElFAD2基因在不同器官中均有表达,且在花后15 d的种子中表达量最高,在叶与花后30 d及45 d种子中的表达量相当,而在根、茎、花中的表达量最低。该研究结果为深入探讨续随子ElFAD2基因的生物学功能提供了基础数据,也为解析续随子种子中脂肪酸合成的分子机制奠定了基础。
中文关键词:续随子  FAD2基因  序列分析  表达特性
 
Sequence and Expression Analysis of ElFAD2 Gene in Euphorbia lathyris
Abstract:In order to study the regulation mechanism of ElFAD2 gene on the synthesis of unsaturated fatty acids in Euphorbia lathyris, this study obtained the ElFAD2 gene sequence based on the transcriptome database, and analyzed its sequence and expression characteristics. Sequence analysis results showed that the full length of ElFAD2 gene was 1 907 bp and the ORF was 1 152 bp, encoding 383 amino acids, containing a typical fatty acid desaturase domain. The isoelectric point of ElFAD2 protein was 8.08 and belonged to a stable protein with 4 transmembrane regions and 3 conserved histidine clusters. The phylogenetic tree analysis showed that E. lathyris had a closer relationship with Triadica sebifera from Euphorbiaceae. Real time PCR analysis revealed that the ElFAD2 gene was expressed in different organs of E. lathyris, but the expression of ElFAD2 gene was the highest in the seeds of 15 days after flowering, and the expression levels are equivalent in the leaves and seeds of 30 days and 45 days after flowering. The expression level was the lowest in roots, stems and flowers. The results facilitated the functional investigation of ElFAD2 gene and provided clues for the analysis of the molecular mechanism of fatty acid synthesis in E. lathyris.
keywords:Euphorbia lathyris  FAD2 gene  sequence analysis  expression profile
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