外源海藻糖对PEG渗透胁迫下小麦Rubisco及其活化酶的影响
    点此下载全文
引用本文:马 超,张 均,宋 鹏,王香生,韩潇杰,冯雅岚.外源海藻糖对PEG渗透胁迫下小麦Rubisco及其活化酶的影响[J].西北植物学报,2019,39(7):1241~1249
摘要点击次数: 0
全文下载次数: 0
作者单位
马 超,张 均,宋 鹏,王香生,韩潇杰,冯雅岚* (河南科技大学 农学院河南省旱地农业工程技术研究中心, 河南洛阳471023) 
基金项目:国家自然科学基金(31401323);
中文摘要:以抗旱品种‘晋麦47’和干旱敏感品种‘郑引1号’为材料,通过室内水培试验研究了外源海藻糖对PEG渗透胁迫下小麦叶片净光合速率、1,5 二磷酸核酮糖羧化酶/加氧酶(Rubisco)和1,5 二磷酸核酮糖羧化酶/加氧酶活化酶(RCA)含量和相关基因表达特性的影响。结果表明:(1)外源海藻糖和渗透胁迫均能显著增加2个小麦品种叶片海藻糖含量。(2)渗透胁迫显著降低了2个品种小麦叶片的净光合速率,而外源海藻糖能显著缓解受胁迫小麦叶片净光合速率的降低幅度。(3)渗透胁迫仅使‘郑引1号’Rubisco大亚基基因(rbcL)相对表达量及相应蛋白含量显著降低;渗透胁迫显著降低了小麦RCA αβ亚基基因相对表达量,并显著降低RCA蛋白含量,而外源海藻糖不能缓解RCA蛋白含量的降低;渗透胁迫显著降低了Rubisco总活性、初始活性、活化状态及RCA活性,而外源海藻糖则能显著缓解上述酶活性的下降。(4)小麦叶片净光合速率与其rbcLRCA αβ亚基基因相对表达量及Rubisco总活性、初始活性、活化状态及RCA活性均呈极显著正相关关系。研究发现,在渗透胁迫条件下,外源海藻糖主要从翻译后层面对小麦叶片Rubisco和RCA的活性发挥显著保护作用,从而缓解了小麦净光合速率的降低。
中文关键词:小麦  渗透胁迫  海藻糖  1,5 二磷酸核酮糖羧化酶/加氧酶  1,5 二磷酸核酮糖羧化酶/加氧酶活化酶
 
Effect of Exogenous Trehalose on Rubisco and Its Activase in Wheat under PEG Osmotic Stress
Abstract:In order to investigate the effects of exogenous trehalose on gene expression of Rubisco and Rubisco activase (RCA) in wheat under osmotic stress, we used the drought resistant variety ‘Jinmai 47’ and the drought sensitive variety ‘Zhengyin 1’ as materials. The results showed that:(1) exogenous trehalose and osmotic stress both could increase the content of trehalose in wheat leaves. (2) Osmotic stress significantly reduced the net photosynthetic rate of wheat leaves, while exogenous trehalose could obviously relieve the decline of the net photosynthetic rate. (3) Relative expression and protein content of Rubisco large subunits (rbcL) were dramatically decreased under osmotic stress in ‘Zhengyin 1’. In addition, osmotic stress observably decreased the relative expression of RCA α and β subunits, as well as the protein content of RCA. However, exogenous trehalose could not alleviate the decline of RCA protein content. Osmotic stress notably reduced Rubisco total activity, initial activity, activation status and RCA activity of. Nevertheless exogenous trehalose could prominently alleviate these indexes. (4)The relevant analysis indicated that the net photosynthesis rate was significantly positively correlated with the relative expression of rbcL, RCA α and β subunits, Rubisco total activity, initial activity, activation status and RCA activity. In conclusion, exogenous application of trehalose plays a substantially protective role in Rubisco and RCA activity from post translational level under osmotic stress, thereby alleviating the decrease of photosynthetic rate.
keywords:wheat  osmotic stress  trehalose  ribulose 1,5 bisphosphate carboxylase/oxygenase(Rubisco)  ribulose 1,5 bisphosphate carboxylase/oxygenase activase(RCA)
查看全文  查看/发表评论  下载PDF阅读器
   今日访问:1060    总访问量:9492328

版权所有:《西北植物学报》编辑部

技术支持:北京勤云科技有限公司