茶树种子发育过程的转录组分析
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引用本文:郑知临,曹红利,王鹏杰,陈 丹,叶乃兴.茶树种子发育过程的转录组分析[J].西北植物学报,2019,39(9):1534~1542
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作者单位
郑知临,曹红利,王鹏杰,陈 丹,叶乃兴* (福建农林大学 园艺学院/茶学福建省高校重点实验室福州 350002) 
基金项目:国家茶叶质量安全工程技术研究中心开放课题基金(2017NTQS0402);
中文摘要:该研究以‘铁观音’茶树品种的种子为试验材料,采用转录组测序技术分析种子发育的3个时期(幼果期、膨大期、成熟期)的表达差异,探究茶树种子油脂代谢的分子机制。结果表明:(1)经转录组测序、组装后共获得30 940 581个clean reads,经数据合并拼接最终得到36 951条非冗余Unigene序列,其中28 476个Unigene可得到功能注释;在转录本中能够被注释到GO分类的Unigene有11 201条(30.3%),KEGG分析发现共有17 172个基因参与了127个代谢通路。(2)经KEGG通路筛选出14条与脂肪酸代谢相关的通路,且随着茶籽的发育,大部分脂肪酸调控途径相关基因呈下调趋势,其中上调基因数最多的有α 亚麻酸代谢途径和脂肪酸降解途径(有17个基因表达量上调),下调基因数最多的是甘油磷脂代谢途径(有58个基因表达量下调);在茶籽发育幼果期α 亚麻酸代谢途径中表达量上调的基因数超过表达量下调的基因数。(3)研究发现茶籽脂肪酸合成相关的基因涉及14个脂类调控途径,共409条差异基因;随着茶树种子发育到成熟期,上调的差异表达基因数量在减少,下调的差异表达基因数量增加,其中α 亚麻酸途径中的基因PLA2G16、DAD1、pldAFabFFabI表达量上调显著,随后表达量下调。(4)qRT PCR检测结果表明,7个茶树FAD和1个ACP差异表达基因的水平与转录组测序结果基本一致;随着茶籽的发育,基因CsFAD7和Δ6 CsFAD从幼果期、果实膨大期至果实成熟期都为差异下调表达,CsFAD2、CsFAD6和Δ7 CsFAD为差异上调表达,CsFAD8、Δ8 CsFADCsACP在幼果期至果实膨大期差异上调表达,在果实膨大期至果实成熟期差异下调表达。
中文关键词:茶树  种子发育  转录组  代谢通路
 
Transcriptomics Analysis of Camellia sinensis Seed in Three Different Development Stages
Abstract:In this study, in order to explore the molecular mechanism of oil metabolism in Camellia sinensis seed, we used transcriptome sequencing to analysis three different stages with young, formation and mature stage of seed development of ‘Tieguanyin’ cultivar. The results indicated that: (1) in total, 30 940 581 clean reads were obtained after transcriptome sequencing and assembly, 36 951 non redundant Unigene sequences were obtained after data assembly, among which 28 476 Unigene could be functionally annotated. 11 201 Unigene (30.3%) were annotated into GO database. 17 172 Unigene were involved in 127 metabolic pathways by KEGG analysis. (2) 14 fatty acid metabolism pathways were selected by KEGG, and with the development of seed, most genes in fatty acid metabolism pathway were down regulated. The number of up regulated genes was the most in the alpha linolenic acid metabolic pathway (17 genes), while the number of down regulated genes was the most in the glycerolipid metabolic pathway (58 genes). More genes were up regulated than down regulated in the alpha linolenic acid metabolism pathway during young fruit stage of tea seeds. (3) A total of 409 differentially expressed genes (DEGs) related to synthesizing of tea seed fatty acid were identified, which were involved in 14 lipid regulation pathways. Some of the genes in the alpha linolenic acid metabolic pathway, like PLA2G16, DAD1, pldA, FabF and FabI, were up regulated significantly, and then the expression was down regulated subsequently. (4) Seven CsFAD genes and one CsACP gene were inspected by qRT PCR and their expression levels were consistent with transcriptome data. With the development of seeds, CsFAD7 and Δ6 CsFAD showed down regulated and CsFAD2, CsFAD6 and Δ7 CsFAD showed up regulated. CsFAD8, Δ8 CsFAD and CsACP showed up regulated from young fruit stage to formation stage,and down regulated from formation stage to mature stage.
keywords:Camellia sinensis  tea seed development  transcriptomics  pathway
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