基于ITS序列西南地区不同居群薤白亲缘关系及其地理分布格局研究
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引用本文:莫忠妹,成 宇,石 甜,赵 财.基于ITS序列西南地区不同居群薤白亲缘关系及其地理分布格局研究[J].西北植物学报,2019,39(9):1573~1580
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作者单位
莫忠妹1,成 宇1,石 甜1,赵 财1,2* (1 贵州大学 生命科学学院贵阳 5500252 贵州大学 山地植物资源保护与种质创新教育部重点实验室贵阳 550025) 
基金项目:贵州省中药材现代产业技术体系建设(GZCYTX 02);
中文摘要:该研究以西南地区分布的薤白(Allium macrostemon Bunge)为材料,对21个不同居群的薤白进行核糖体DNA的 ITS序列测定,并结合GenBank相关序列,对27个不同居群薤白开展了系统分析,以探讨西南地区不同居群薤白的亲缘关系和生物地理分布格局。结果表明:(1)西南地区不同产区薤白nrDNA ITS序列长度介于607~638 bp之间,G+C含量为48%~52%,平均值为50%。(2)基于贝叶斯法和最大简约法构建的系统树均表明薤白为一单系群。(3)系统发育树分析结果显示,27个居群的薤白总体划分为a组和b组,其中位于西南地区东面的四川剑阁县、重庆巫溪县、贵州德江县和云南丘北县的薤白共同聚为a组,位于西南地区西面的四川汶川县、四川石棉县、四川江油市以及云南虎跳峡、云南昆明市、云南三坝的薤白归为b组。研究表明,nrDNA ITS序列可以鉴定不同地区薤白的亲缘关系。
中文关键词:薤白  nrDNA ITS序列  亲缘关系,西南地区
 
Genetic Relationship and Geographical Distribution Pattern of Different Allium macrostemon Populations in Southwest China Based on ITS Sequences
Abstract:To explore the relationship and biogeographic distribution pattern of A. macrostemon in different areas of Southwest China, we compared and analyzed 27 nrDNA ITS sequences of A. macrostemon, of which 21 were newly generated and 6 from GenBank. The results showed: (1) the sequence length of the A. macrostemon Bunge varied from 607-638 bp and the GC content of 48%-52%, with an average 50%. (2) A. macrostemon is monophyletic basing on Bayesian inference and Maximum parsimony analysis. (3) Phylogenetic analysis indicated that the 27 populations are generally divided into group a and group b. Among them, Jiange County, Sichuan, Wixi County, Chongqing, Dejiang County, Guizhou and Qiubei County, Yunnan, located in the east of Southwest China, are clustered into group a; Wenchuan, Shimian, Jiangyou of Sichuan Province and Hutiaoxia, Kunming, Sanba of Yunnan Province located in the west of Southwest China belongs to group b. The results suggested that nrDNA ITS sequence could identify the relationship of A. macrostemon in different regions.
keywords:Allium macrostemon Bunge  nrDNA ITS sequence  relationship  southwest region
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