文心兰FT同源基因的克隆及其表达分析
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引用本文:林榕燕,方能炎,罗远华,黄敏玲,叶秀仙,钟淮钦.文心兰FT同源基因的克隆及其表达分析[J].西北植物学报,2019,39(10):1718~1724
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林榕燕,方能炎,罗远华,黄敏玲,叶秀仙,钟淮钦* (福建省农业科学院 作物研究所花卉研究中心福建省特色花卉工程技术研究中心福州 350013) 
基金项目:福建省自然科学基金(2017J01063);
中文摘要:FT (Flowering Locus T)及其同源基因被认为是植物开花、花发育相关的重要基因。为了深入研究FT 同源基因的功能以及文心兰开花的分子机理,该研究以文心兰品种‘金辉’为试验材料,基于转录组测序结果,克隆获得‘金辉’FT 同源基因,命名为OnFT(GenBank登录号为MK967676)。OnFT基因编码区长度为537 bp,共编码178个氨基酸;生物信息学分析表明,该蛋白质分子量约为19.99 kD,可能是一种亲水性不稳定蛋白质,属于PEBP家族蛋白;基于FT的系统进化分析表明,文心兰与同科植物桃红蝴蝶兰的亲缘关系较近。qRT PCR分析结果显示,OnFT在文心兰不同组织的表达差异较大,在花中表达量最高,在根中几乎不表达;OnFT基因在幼嫩花芽中的表达量较低,并且在花的成熟过程中逐渐增加;OnFT基因在叶片和假鳞茎中的表达量随成熟度的增长均呈先上升后降低的变化趋势,在中苗期的叶片和抽芽期的假鳞茎中表达量较高。
中文关键词:文心兰  FT同源基因  克隆  qRT PCR
 
Cloning and Expression Analysis of FT Homologous Gene from Oncidium ‘Jinhui’
Abstract:FT (Flowering Locus T) and its homologous gene were considered to be important genes which related to plants blossom and flower development. To better study the function of the FT homologous gene and flowering mechanism of Oncidium ‘Jinhui’, we isolated the FT homologous gene named OnFT and its GenBank accession number was MK967676 on the basis of transcriptome sequencing results in this study. OnFT contains a 537 bp open reading frame which encoding 178 amino acids. Bioinformatic analysis showed that the molecular weight of OnFT protein was 19.99 kD and this protein might be a unstable hydrophilic protein. Besides, the OnFT protein belonged to the PEBP family. The phylogenetic analysis showed that Oncidium was closely related to Phalaenopsis equestris from Orchidaceae. qRT PCR results indicated that the relative expression of OnFT gene were different in four tissues, with the highest expression was in flowers and almost no expressed in root. The expression level of OnFT gene in young flower buds was low and increased gradually during flower maturation. The expression of OnFT gene in leaves and pseudobulbs increased first and then decreased with the growth of maturity, highly expressed in leaves of middle seedling stage and pseudobulbs of budding stage.
keywords:Oncidium  FT homologous gene  cloning  qRT PCR
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