Abstract:We used the Chinses old garden rose Rosa chinensis ‘Old Blush’ as test material and designed the degenerate primers according to the conserved domains of the FT/TFL1 gene family members of Arabidopsis thaliana and Fragaria vesca. We cloned and sequenced the genomic DNA of R. chinensis ‘Old Blush’, and did the sequence alignment based on database sequence and double haploid genomic information. We obtained a total of 8 FT/TFL1 gene family sequences, and the 8 sequences were respectively annotated as 2 FT genes (RcFT1 and RcFT2), 1 BFT gene (RcBFT), 1 ATC gene (RcATC), 2 MFT genes (RcMFT1 and RcMFT2), and 2 PEBP genes (PEBP1 and PEBP2). Phylogenetic tree analysis divided these 8 genes into 3 subgroups. Structural analysis showed that the introns of 8 genes ranged from 1-5. qRTPCR expression analysis FT/TFL1 genes in different tissues of R. chinensis ‘Old Blush’ showed that RcFT1, RcPEBP, RcMFT1, RcMFT2 and RcBFT have the highest expression in leaves, followed in stem tips. RcFT2 had the highest expression level in stem tips and second in leaves. The expression of RcATC in stem tips was the highest, while lower in other tissues. RcPEBP gene has expression in root tissues, other genes have no expression in roots. In addition, the expression level of RcFT1 and RcFT2 in the leaves and stem tips of R. wichuriana ‘Basyes Thornless’ during the reproductive growth period were significantly higher than those during the vegetative growth period. We speculated that the R. chinensis ‘Old Blush’ with continuous flowering may have multiple flowering time (FT) gene loci, and FT may be used as a marker gene for reproductive transformation of rose.