黑粒青稞HvtUF3GT基因的克隆与表达分析
    点此下载全文
引用本文:苏乐平,姚晓华,安立昆,吴昆仑,迟德钊.黑粒青稞HvtUF3GT基因的克隆与表达分析[J].西北植物学报,2019,39(10):1750~1757
摘要点击次数: 0
全文下载次数: 0
作者单位
苏乐平1,姚晓华1,2,3,4,安立昆1,2,3,4,吴昆仑1,2,3,4,迟德钊1,2,3,4* (1 青海大学西宁 8100162 青海省农林科学院西宁 8100163 青海省青稞遗传育种重点实验室西宁 8100164 国家麦类改良中心青海青稞分中心西宁 810016) 
基金项目:国家大麦产业技术体系(CARS 05);
中文摘要:该研究以黑粒青稞品种‘黑老鸦’籽粒为材料,克隆了青稞类黄酮3 O 葡萄糖基转移酶基因(HvtUF3GT),获得1 449 bp序列。HvtUF3GT基因包含一个1 434 bp开放阅读框,编码478个氨基酸,理论等电点为5.45,预测蛋白分子量为52 912.58 Da。蛋白质序列分析表明,HvtUF3GT为亲水性的不稳定酸性蛋白,且在C 末端具有一段典型的44个氨基酸PSPG box,在空间结构中含有β α β α β Rossmann折叠结构,属于UDP糖基转移酶基因GT B型超家族成员。进化树分析表明,HvtUF3GT与小麦族的大麦、乌拉尔图小麦、山羊草和二穗短柄草的糖基转移酶聚为一个分支, 且与大麦的亲缘关系最近,与稻族的籼稻和短花稻的亲缘关系较远。半定量和定量PCR结果表明,在‘INB0N 7’蓝粒青稞和‘昆仑12号’白粒青稞的籽粒灌浆后期HvtUF3GT基因不表达,而在黑粒青稞和紫粒青稞中的表达强弱依次为‘黑老鸦’(黑粒)>‘昆仑17号’(黑粒)>‘达章紫’(紫粒)>‘涅如姆扎’(紫粒)。研究推测,HvtUF3GT基因在青稞灌浆后期籽粒着色过程中发挥着重要作用。
中文关键词:青稞  花青素  HvtUF3GT基因  基因表达
 
Isolation and Expression Analysis of HvtUF3GT Gene in Black Tibetan Hulless Barley
Abstract:In this study, we isolated the flavonoid 3 O glucosyltransferase gene named HvtUF3GT from ‘Heilaoya’ grain, and obtained a 1 449 bp sequence. HvtUF3GT gene contained a 1 434 bp open reading frame (ORF), encoding 478 amino acids. The theoretical isoelectric point (pI) was 5.45 and the predicted protein molecular weight was 52 912.58 Da. Protein sequence analysis showed that HvtUF3GT was a hydrophilic unstable acidic protein containing a typical 44 amino acids PSPG box at the C terminus and a β α β α β Rossmann folded structure in the spatial structure. HvtUF3GT was a member of UDP glycosyltransferase_GTB _type superfamilies. Phylogenetic tree analysis showed that HvtUF3GT and the glycosyltransferase of Trib. Triticeae such as Hordeum vulgareTriticum urartuAegilops tauschii and Brachypodium distachyon were clustered into one branch. HvtUF3GT was closely related to H. vulgare, while far away from Oryza sativa (indica rice) and Oryza brachyantha belonging to Trib. Oryzeae. Semi quantitative and quantitative PCR results showed that the HvtUF3GT gene was not expressed in‘INB0N 7’with blue grain coat and‘Kunlun 12’ with white grain coat,while the expression level in the grain of black and purple coat was‘Heilaoya’ (black grain)>‘Kunlun 17’ (black grain)> ‘Dazhangzi’ (purple grain)> ‘Nierumuzha’ (purple grain) during the late stage of grain grouting in Tibetan hulless barley. Thus,it was speculated that the HvtUF3GT gene could play an important role in the process of grain coloration in Tibetan hulless barley.
keywords:Tibetan hulless barley  anthocyanins  HvtUF3GT  expression level
查看全文  查看/发表评论  下载PDF阅读器
   今日访问:1867    总访问量:9836728

版权所有:《西北植物学报》编辑部

技术支持:北京勤云科技有限公司