草莓果实线粒体呼吸代谢与超微弱发光的关系
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引用本文:孙 聪,白 杨,李连国,郭金丽.草莓果实线粒体呼吸代谢与超微弱发光的关系[J].西北植物学报,2019,39(10):1805~1811
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孙 聪,白 杨,李连国,郭金丽* (内蒙古农业大学 园艺与植保学院,呼和浩特 010010) 
基金项目:国家自然科学基金(31260455)
中文摘要:该试验以草莓(Fragaria ananassa Duch.)品种‘红颜’果实为试材,采用水杨基氧肟酸(SHAM)抑制线粒体交替呼吸途径,使细胞色素途径单独运行,并用电子传递与氧化磷酸化偶联促进剂[二磷酸腺苷(ADP)、琥珀酸钠(C4H4Na2O4)]和抑制剂[2,4 二硝基苯酚(DNP)、原钒酸钠溶液(NaVO4)]对线粒体提取液进行处理,对比分析在促进和抑制呼吸代谢情况下,草莓果实线粒体呼吸代谢与超微弱发光(UWL)的变化及二者之间的关系。结果显示:(1)草莓果实线粒体经促进剂处理后,呼吸代谢关键酶琥珀酸脱氢酶(SDH)、细胞色素氧化酶(COX)、ATP合酶(H+ ATPase)活性以及呼吸速率、三磷酸腺苷(ATP)含量均随着促进剂浓度的增加而增加,UWL强度亦增强,且各浓度下的呼吸代谢指标和UWL强度均高于对照;经抑制剂处理后,以上呼吸代谢各指标及UWL强度的变化与促进剂处理相反,且均低于对照。(2)各促进剂、抑制剂处理条件下,草莓果实线粒体呼吸代谢各指标均与其UWL强度呈正相关。研究表明,草莓果实线粒体UWL强度随着呼吸代谢的变化而变化;促进剂促进了呼吸代谢,导致UWL强度增加,而抑制剂却抑制了呼吸代谢,导致UWL强度减弱;线粒体是UWL产生的细胞器之一,线粒体呼吸代谢过程中激发了UWL。
中文关键词:草莓果实  线粒体  呼吸代谢  超微弱发光
 
Relationship between Mitochondrial Respiratory Metabolism and Ultraweak Luminescence in Strawberry Fruit
Abstract:In this experiment, the‘Hongyan’strawberry fruit (Fragaria ananassa Duch.) was used as test material, and salicylhydroxamic acid (SHAM) was used to inhibit the mitochondrial alternative pathway to make the cytochrome pathway run alone. The mitochondrial extract was treated with electron transfer and oxidative phosphorylation coupling accelerators [adenosine diphosphate (ADP) and succinic acid disodium salt (C4H4Na2O4)] and inhibitors [2,4 dinitrophenol (DNP) and sodium vanadate solution (NaVO4)]. Under the condition of promoting and inhibiting respiratory metabolism, the changes of mitochondrial respiratory metabolism and ultra weak luminescence (UWL) and the relationship between them were compared and analyzed in strawberry fruit.The results showed that: (1) the mitochondria of strawberry fruit were treated with accelerator, the key enzymes activity of respiratory metabolism including succinate dehydrogenase (SDH), cytochrome oxidase (COX), ATP synthase (H+ ATPase), respiration rate and adenosine triphosphate (ATP) content increased with the increase of concentration, and the intensity of UWL increased with the increase of concentration. The respiratory metabolic index and UWL intensity at each concentration were higher than those of the control. After treatment with inhibitor, the changes of respiratory metabolism indexes and UWL intensity were opposite to those of accelerator, and all of them were lower than those of the control. (2) The correlation analysis showed that all indexes of respiratory metabolism were positively correlated with UWL.The above results showed that the mitochondrial UWL intensity of strawberry fruit changed with the change of respiratory metabolism; the accelerator promoted the respiratory metabolism and led to the increase of UWL intensity; on the contrary, the inhibitor inhibited respiratory metabolism and led to the decrease of UWL intensity; It suggested that mitochondria was one of the organelles that produced UWL, and UWL was stimulated in the process of mitochondrial respiration and metabolism.
keywords:strawberry fruit  mitochondria  respiratory metabolism  ultraweak luminescence
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