茶丽纹象甲为害诱导的茶树防御反应分析
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引用本文:高香凤,李慧玲,王让剑.茶丽纹象甲为害诱导的茶树防御反应分析[J].西北植物学报,2019,39(11):1943~1951
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作者单位
高香凤,李慧玲,王让剑* (福建省农业科学院 茶叶研究所福建福安 355015) 
基金项目:国家重点研发计划(2016YFD0200902);
中文摘要:基于转录组测序,获得了茶树新梢叶片被茶丽纹象甲为害前后的差异基因表达谱,并从信号监测与转导、转录因子、次生代谢物生成等方面分析了相关基因的表达变化情况,为进一步研究茶丽纹象甲为害诱导的茶树防御机制奠定基础。结果表明:(1)茶丽纹象甲为害茶树新梢叶片后共检测到显著上调表达基因309个,显著下调表达基因297个,这些显著差异表达基因共分成23类。(2)检测到信号监测与转导过程中合计17个单基因上调表达2.0~2.8倍,包括富含亮氨酸重复序列的类受体蛋白激酶基因、促分裂原活化蛋白激酶级联信号系统蛋白激酶基因、茉莉酸信号通路基因、钙离子信号基因、水杨酸代谢通路基因;检测到21个转录因子单基因上调表达1.8~2.8倍,包括bHLH、WRKY、bZIP、MYB、UAF等转录因子;检测到苯丙类、类黄酮及萜类物质代谢过程中合计8个单基因上调表达2.1~2.5倍,包括苯丙酮酸互变异构酶基因、肉桂酰辅酶A还原酶基因、二氢黄酮醇还原酶基因、花色素还原酶基因、法尼基二磷酸合成酶基因、法尼醇脱氢酶基因。研究认为,茶丽纹象甲为害诱导信号转导、转录因子及次生代谢过程中的基因增量表达以机械损伤作用为主,昆虫口腔分泌物对这些基因的诱导表达具有协同促进作用。
中文关键词:茶树  茶丽纹象甲  信号转导  转录因子  次生代谢  差异基因表达谱  转录组测序
 
Defense Response of Tea Plants Induced by Myllocerinus aurolinealus Voss Damage
Abstract:Based on transcriptome sequencing, we obtained the differential gene expression profiles of tea shoot leaves undamaged and damaged by Myllocerinus aurolinealus Voss, and the expression differences of related genes correlated with signal monitoring and transduction, transcription factors, and secondary metabolites biosynthesis were analyzed. The study was capable of providing a reference for further research on the defense mechanism of tea plants induced by weevil damage. The results showed that: (1) 309 up regulated and 297 down regulated genes were detected after tea weevil damaged, and were divided into 23 groups. (2) 17 unigenes were up regulated by 2.0-2.8 folds in signal monitoring and transduction process, including leucine rich repeat receptor like kinase gene, mitogen activated protein kinase gene, jasmonic acid signal pathway gene, calcium ion signal gene and salicylic acid metabolism pathway gene. 21 transcription factors were up regulated by 1.8-2.8 folds, including bHLH, WRKY, bZIP, MYB and UAF. Eight unigenes in the metabolism of phenylpropanoids, flavonoids and terpenoids were up regulated by 2.1-2.5 folds, including phenylpyruvate tautomerase gene, cinnamoyl CoA reductase gene, dihydroflavonol reductase gene, anthocyanidin reductase gene, anthocyanidin reductase gene, farnesol dehydrogenase gene. The increased gene expression induced by weevil feeding is mainly caused by mechanical damage, and the insect oral secretions have synergistic effects on the up regulated of these genes.
keywords:Camellia sinensis  Myllocerinus aurolinealus Voss  signal transduction  transcription factor  secondary metabolism  differential gene expression profiles  RNA sequencing
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