建兰水晶艺叶片的超微结构和转录组学分析
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引用本文:艾 叶,陈 娟,郑清冬,陈明堃,马山虎,彭东辉.建兰水晶艺叶片的超微结构和转录组学分析[J].西北植物学报,2020,40(3):382~393
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作者单位
艾 叶,陈 娟,郑清冬,陈明堃,马山虎,彭东辉* (福建农林大学 园林学院兰科植物保护与利用国家林业与草原局重点实验室福州 350002) 
基金项目:国家自然科学基金(31700618);
中文摘要:水晶艺建兰(Cymbidium ensifolium)因其叶片上呈现出白色透明状,犹如水晶而得名,观赏价值高,但是其形成机理不明确。该研究以建兰‘铁骨水晶’为试验材料,通过对水晶叶片和绿色叶片进行显微结构和超微结构观察,并结合转录组测序等方法,探索建兰水晶艺叶片形成的原因。结果表明:(1)建兰‘铁骨水晶’水晶叶片比绿色叶片薄,叶肉细胞数量减少,形状不规则,且叶绿体含量少;水晶叶片的表皮气孔数量较绿色叶片显著减少;水晶叶片的叶肉细胞中叶绿体结构发育不良,叶绿体双膜和类囊体膜模糊,细胞中存在着大量的嗜锇颗粒。(2)转录组数据分析显示,水晶叶片中与光合作用 天线蛋白、光合作用等代谢途径相关的基因表达量显著下降,而与色素合成代谢途径相关的基因表达量上升。研究推测,建兰水晶艺叶形成的原因可能是由于与光合作用相关的基因表达量降低,导致叶绿体发育不良,叶绿素合成受阻,从而形成白色透明状叶片。
中文关键词:建兰  叶色突变体  转录组测序  光合作用
 
AI Ye, CHEN Juan, ZHENG Qingdong, CHEN Mingkun, MA Shanhu, PENG Donghui*
Abstract:Crystal leaf mutants of Cymbidium ensifolium have high ornamental value due to their leaves being white transparent shape, but their formation mechanism is not clear. Taking C. ensifolium ‘Tiegushuijing’ as the material, this study intends to explore the reasons for the formation of crystal leaf mutants of C. ensifolium by observation of microstructure and ultrastructure and RNA seq. The results showed that: (1) the crystal leaves of C. ensifolium ‘Tiegushuijing’ were thinner than the green leaves, the number of mesophyll cells was significantly reduced, the cell shape was irregular, and the chloroplast content was small, a significant reduction in the number of stomata in the epidermis of the crystal leaf than the green leaves; the chloroplast structure of mesophyll cells in crystal leaves was poorly developed, the chloroplast double membrane and thylakoid membrane were blurred, and there were a large number of osmiophilic granule in the cells. (2) The RNA seq data results showed that the expression levels of genes related to photosynthesis antennae and photosynthesis metabolic pathways were decreased in crystal leaves, while the expression levels of genes related to pigment metabolic pathways were increased. We speculated that the reason for the formation of crystal leaves of C. ensifolium is that the expression of genes related to photosynthesis are reduced, the chloroplast is poorly developed, and the chlorophyll synthesis is blocked, thereby forming white transparent leaves.
keywords:Cymbidium ensifolium  leaf color mutant  RNA seq  photosynthesis
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