PEG模拟干旱胁迫下马铃薯茎段转录组分析
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引用本文:赵 龙,王 舰,王 芳.PEG模拟干旱胁迫下马铃薯茎段转录组分析[J].西北植物学报,2020,40(3):403~412
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作者单位
赵 龙1,王 舰1,2,3,4,5,王 芳1,2,3,4,5* (1 青海大学西宁8100162 青海省农林科学院西宁8100163 青海大学省部共建三江源生态与高原农牧业国家重点实验室西宁8100164 青藏高原生物技术教育部重点实验室西宁8100165 青海省马铃薯育种重点实验室西宁810016) 
基金项目:国家自然科学基金(31660417);
中文摘要:该研究以‘青薯9号’马铃薯无菌苗为材料,采用转录组测序技术分析模拟干旱胁迫下马铃薯茎段的差异表达,探究茎段在干旱胁迫下的分子机制。结果表明:(1)不同程度干旱胁迫下,马铃薯叶片脯氨酸、可溶性糖以及可溶性蛋白含量明显增加;马铃薯茎段差异表达基因下调的数量均多于上调,其中3种处理条件下共有的差异表达基因有657个。(2)GO富集分析表明,马铃薯茎段差异表达基因主要集中在氧化还原过程、激素响应、氧化还原酶活性以及糖基水解酶活性;Pathway富集分析表明,马铃薯茎段差异表达基因主要集中在植物激素信号转导、苯丙酸生物合成、玉米素生物合成、苯丙氨酸代谢、淀粉和蔗糖代谢以及次生代谢产物的生物合成。(3)实时荧光定量PCR验证结果表明,6个差异表达基因在不同程度干旱胁迫中的差异表达与转录组分析的结果基本一致,证明转录组数据的可靠性。该结果对进一步研究马铃薯干旱胁迫响应机制有一定参考价值,也丰富了马铃薯抗旱育种的基因资源。
中文关键词:马铃薯  干旱胁迫  转录组分析  差异表达基因
 
Transcriptome Analysis of Potato Stem under Drought Stress Simulated by PEG
Abstract:In this paper, we studied the plantlets of potato variety ‘Qingshu 9’ under simulated drought stress to explore the molecular mechanism of potato stem under drought stress, respectively, and the stems of plantlets were used for transcriptome sequencing. The results showed that: (1) the contents of proline, soluble sugar and soluble protein were increased with the increasing concentration of PEG6000. Under three different drought stressed conditions, there were more down regulated candidate genes than up regulated candidate genes with a total of 657 differential expression genes (DEGs) in potato stem. (2) GO enrichment analysis showed that the DEGs in potato stems mainly focused on oxidation reduction process, response to hormone, oxidoreductase activity, glycosylhydrolase activity. KEGG pathway enrichment analysis showed that the DEGs in potato stems mainly involved in plant hormone signal transduction, phenylpropanoid biosynthesis, zeatin biosynthesis, phenylalanine metabolism, starch and sucrose metabolism, biosynthesis of secondary metabolites. (3) Real time fluorescence quantitative PCR (qPCR) was used to verify the expressions of six DEGs under different drought stress conditions. The results were basically consistent with the transcriptome profile that proved the reliability of our RNA seq data. Our research provided the valuable information for understanding the drought resistance mechanism of potato, and enriching the genetic resources of drought resistant potato breeding.
keywords:potato  drought stress  transcriptome analysis  DEGs
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