基于cpDNA非编码序列的北沙柳遗传多样性分析
    点此下载全文
引用本文:李娅翔,路东晔,郝 蕾,张国盛,宁 静,乌兰娜日,阿拉腾苏和.基于cpDNA非编码序列的北沙柳遗传多样性分析[J].西北植物学报,2020,40(3):413~424
摘要点击次数: 42
全文下载次数: 46
作者单位
李娅翔1,路东晔1,郝 蕾2,张国盛1*,宁 静3,乌兰娜日4,阿拉腾苏和5 (1 内蒙古农业大学 林学院呼和浩特 0100192 内蒙古财经大学呼和浩特 0100703 内蒙古林业科学研究院呼和浩特 0100104 鄂尔多斯乌审旗林业局内蒙古 达布察克 0173005 鄂尔多斯造林总场内蒙古 树林召 014300) 
基金项目:内蒙古自治区自然科学基金(2018MS03013)
中文摘要:为揭示北沙柳(Salix psammophila)的遗传多样性、遗传结构及分化特征,利用叶绿体非编码区序列(trnL trnFtrnD trnT)对分布于毛乌素沙地和库布齐沙漠的16个北沙柳居群(339个个体)进行了遗传研究,为北沙柳种子资源库遗传管理、遗传改良、遗传育种及品种选育提供理论依据。结果表明:(1)经trnL trnFtrnD trnT片段联合比对获得了1 811 bp序列,共有12个核苷酸变异位点(8个简约信息位点,4个变异位点),得到16个单倍型。(2)单倍型多样性指数(Hd)为0.737,核苷酸多样性指数(π)为0.001 07;且单倍型H3的分布在所有居群中位于单倍型网络图中心,其余单倍型随机分布于各个居群。(3)AMOVA分析表明,北沙柳cpDNA的变异主要来源于居群内(91.16%),居群间遗传分化程度中等(FST=0.088 37),各居群间的基因交流非常频繁(Nm =2.58);遗传分化系数 NST(0.085)显著大于 GST(0.056,0.01< P <0.05),表明北沙柳居群具有明显的分子谱系地理结构。(4)中性检验及失配分析表明,北沙柳居群分布范围没有发生明显扩张的痕迹。
中文关键词:北沙柳  cpDNA  遗传多样性  单倍型  遗传结构
 
Genetic Diversity of Salix psammophila Based on cpDNA Non coding Sequence
Abstract:We used non coding chloroplst DNA sequence (trnL trnF and trnD trnT) to study the genetic diversity of 16 populations of Salix psammophila distributed in Mu Us Sandy land and Kubuqi deserts,which aimed to explore genetic diversity, structure and differentiation characteristics of this species. This study provide theoretical basis for genetic management, improvement, breeding and variety selection of S. psammophila. The main results showed that: (1) the length of combine sequences was 1 811 bp, a total of 12 nucleotide mutation sites were identified and 16 haplotypes were recovered, including 8 simple information sites and 4 mutation sites. (2) The haplotype diversity was 0.737. The nucleotide diversity was 0.001 07. Only haplotype H3 located in the center of the haplotype network map distributed in all populations, while other haplotypes were randomly distributed among the populations. (3) AMOVA analysis suggest that 91.16% of the total variation existed within population while among population variation was medium (FST = 0.088 37). The exchanging of gene between the populations was very frequent (Nm=2.58). There is significant phylogeographic structure in population of S. psammophila. (NST=0.085, GST=0.056, 0.01 < P < 0.05). (4) Neutral tests and mismatch analysis proved that the distribution of S. psammophila population did not expand significantly.
keywords:Salix psammophila  chloropast DNA  genetic diversity  haplotypes  genetic structure
查看全文  查看/发表评论  下载PDF阅读器
   今日访问:3614    总访问量:11091470

版权所有:《西北植物学报》编辑部

技术支持:北京勤云科技有限公司