茶树种子发育过程的转录组分析
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福建农林大学园艺学院/茶学福建省高校重点实验室

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国家茶叶质量安全工程技术研究中心开放课题基金(2017NTQS0402);福建省“2011协同创新中心”中国乌龙茶产业协同创新中心专项(闽教科〔2015〕75号);福建农林大学科技创新专项基金项目(CXZX2017181),


Transcriptomics Analysis of Camellia sinensis Seed in Three Different Development Stages
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National Tea Quality and Safety Engineering Technology Research Center Open Project Fund (2017NTQS0402); Fujian Province “2011 Collaborative Innovation Center” China Oolong Tea Industry Collaborative Innovation Center Special (闽教科 [2015] No. 75); Fujian Agriculture and Forestry University Science and Technology Innovation Special Fund Project (CXZX2017181 ),

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    摘要:

    以‘铁观音’品种的种子为试验材料,采用转录组测序技术分析种子发育的3个时期(幼果期、膨大期、成熟期)的表达差异,探究茶树种子油脂代谢的分子机制。研究结果中发掘出茶籽脂肪酸合成相关的基因涉及14个脂类调控途径,共409条差异基因,并且α-亚麻酸代谢途径中上调基因数最多,其中PLA2G16、DAD1、pldA、FabF、FabI表达量上调显著。qRT-PCR检测了7个茶树FAD和1个ACP差异表达基因的表达水平,检测结果与转录组测序结果基本一致。本研究为深入开展茶籽脂肪酸合成相关基因的克隆和功能验证提供了丰富的信息,并为揭示茶籽脂肪酸合成机制提供了帮助。

    Abstract:

    To explore the molecular mechanism of oil metabolism in Camellia sinensis seed, transcriptome sequencing was used to analysis three different stages with young, formation and mature stage of seed development of "Tieguanyin" cultivar. The results indicated that a total of 409 differentially expressed genes(DEGs)related to synthesising of tea seed fatty acid wereidentified, which were involved in 14 lipid regulation pathways. The number of up-regulated genes was the most in the alpha linolenic acid metabolic pathway,of which PLA2G16、DAD1、pldA、FabF、FabI were up-regulated significantly. Seven CsFAD genes and one CsACP gene, were inspected by qRT-PCR and their expression levels were consistent with transcriptome data. This study provides a wealth of information for further cloning and functional verification of tea seed fatty acid synthesis related genes, and provides assistance for revealing the mechanism of tea seed fatty acid synthesis.

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  • 收稿日期:2019-05-28
  • 最后修改日期:2019-07-19
  • 录用日期:2019-07-23
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