Petunia hybrida var. Mitchel diploid was used as the experimental material in this study. PhUGT74E2, the homologous gene of UGT74E2 was cloned. The full length of PhUGT74E2 was 1347 bp, encoding 448 amino acids. The molecular formula of the protein was speculated to be C2278H3544N586O676S18. Phylogenetic tree analysis showed that PhUGT74E2 had the same origin as other species, but was most closely related to tobacco NtUGT74E2. The promoter sequence of 2083 bp upstream of PhUGT74E2 gene was cloned. The sequence contains abscisic acid, gibberellin, light and stress response elements. Fluorescence quantitative PCR analysis showed that PhUGT74E2 gene was expressed in leaves, stems, roots, axil and apical. The expression level of PhUGT74E2 in axil was highest, while lowest in apical. After 12 h of PEG 6000 treatment, the expression level of PhUGT74E2 increased to 40.8 times that of the control group and 42.8 times at 24 h. After 12 h of NaCl treatment, the expression level of PhUGT74E2 increased to 37.6 times that of the control group and 38.4 times at 24 h. This indicated that PhUGT74E2 was involved in drought and salt stress response of petunia. This study laid a theoretical foundation for further research on the function of PhUGT74E2 and the molecular mechanism of PhUGT74E2 regulating the stress tolerance of petunia.