The ZF-HD (Zinc finger homeodomain) protein family is one kind of plant specific transcription factor family which plays important roles during stress response, flower development and seeds longevity of plant, but their function in strawberry is unknown. This study firstly identified 10 genes of ZF-HD family from woodland strawberry and divided them into 6 subfamilies by analysis of the evolutionary relationship. By analysis the cis-element of the promoter, we find that there are many of the cis-element which related to the adversity stress and hormone response. We checked their expression level in flowers and find that FvZF-HD7 gene was expressed more abundant compared with other genes. Further more, the tissue organ specific expression analysis indicted that this gene is expressed highest in the flowers, but also expressed in leaves and stem. During fruit development, it is highly expressed in the earlier stage but decreased rapidly in the later stage. The result indicated that FvZF-HD7 may widely take part in the regulation of flower development and stress response. The gene was successfully cloned by using the cDNA of woodland strawberry ‘Yellow Wonder 5AF7’ (YW).The bioinformatics analysis suggested that the full length of this gene is 1 083 bp, encoding 360 amino acid. The sequence aligment indicate that the edcoded protein possesses both ZF and HD conserved domains. The molecular weight of the protein is expected to be 39 153.89 Da with abundant α-helix and random coil. The subcellular localization analysis confirmed that this protein exclusively localizes in the nucleus. This research laid the theoretical and molecule foundation for studying the function of ZF-HD in woodland strawberry.