NaCl胁迫对流苏幼苗生长、Na+和K+分布及渗透调节物质的影响
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南京林业大学

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国家林业局“948”项目(2014-4-17);江苏高校品牌专业建设工程资助项目(PPZY2015.A063)


Effects of NaCl Stress on Growth,Na+ and K+ distribution and Osmotic Regulation of Chionanthus retusue Seedlings
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    摘要:

    以2年生的流苏播种苗为材料,采用不同浓度(50、100、200、300mmol?L-1)NaCl溶液进行胁迫处理,研究盐胁迫对流苏的生长、Na+和K+分布格局、渗透调节物质的影响,探讨了耐盐阈值。结果表明:随着NaCl浓度的增加,流苏幼苗生长量逐渐降低,盐害指数升高、存活率下降;耐盐阈值为98.693mmol?L-1(0.577% w/v);流苏各器官中的Na+含量一直增加,50mmol?L-1时,Na+含量根>叶>茎,其余各处理组Na+含量叶>根>茎;根、叶中K+含量表现为先增后减的变化,茎中K+含量总体表现为下降趋势,K+含量根>叶>茎;由根部到茎部,由茎部到叶部SK,Na、各器官中K+/Na+比值呈下降的变化;随着NaCl浓度的增加,叶片可溶性糖、可溶性蛋白含量总体呈上升趋势;脯氨酸含量呈先上升后下降趋势。根系可通过对Na+的吸收和累积来阻止其向地上部运输进而避免盐害发生;叶片和茎中通过提高对K+的选择性吸收和累积,从而增大K+/Na+比值以减缓盐分对其生理代谢的伤害。

    Abstract:

    in order to exploit the salt-tolerance ability of Chionanthus retusue,The plant growth, distributionpattern of Na+ and K+ and relative conductivity and osmotic adjustment substances of Chionanthus retusuewere studied under different concentrations(50, 100, 200, 300 mmol?L-1) of NaCl solution with potted two-year seedlings. The results show that with the increase of NaCl concentration, the seedling growth of Chionanthus retusue decreased gradually, the salt damage index increased and the survival rate decreased. Salt tolerance threshold was 98.693 mmol?L-1 (0.577% w/v). The content of Na+ in every organ of Chionanthus retusue increased continuously. At 50mmol?L-1, Na+ content in root > leaf > stem, and Na+ content in other treatments was leaf > root > stem. The content of K+ in roots and leaves increased first andthen decreased. The content of K+ in stems decreased in general. The content of K+ in roots > leaves > stems was higher than that in leaves. The ratio of K+/Na+ in SK,Naand organs decreased from root to stem,from stem to leaf. With the increase of NaCl concentration, the content of soluble sugar and soluble protein in leaves increased in general; the proline content showed an upward trend and then a downward trend. It is concluded that under Salt Stress. The root system of Chionanthus retusue seedlings can balance the damage of salt stress by enlarging the root growth; the root system can prevent the salt damage by absorbing and accumulating Na+ to the above-ground parts; the leaf and stem can increase the selective absorption and accumulation of K+, thereby increasing the ratio of K+/Na+ to slow down the physiological metabolism of salt damage.

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  • 收稿日期:2019-11-08
  • 最后修改日期:2020-02-10
  • 录用日期:2020-02-03
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