Abstract:To study the molecular mechanism of photosynthesis, a wheat Lhca gene was cloned from the leaves of ‘Bainong207’ using RT-PCR, and was designated as TaLhca. The coding sequence (CDS) of this cloned Lhca gene is 810 bp in length, encoding a polypeptide of 269 amino acids. The deduced protein molecular weight was 29.31 kD and its theoretical isoelectric point was 8.69. Bioinformatics analysis showed that TaLhca is a hydrophilic non-secretory protein with three transmembrane domains and a chlorophyll a/b binding domain. Multiple sequence alignments and phytogenetic analysis of Lhca proteins showed TaLhca is most similar with Lhca from Brachypodium distachyon and Oryza sativa. Cis-regulatory elements analysis revealed that light responsiveness elements, stress responsiveness elements was in the promoter region of TaLhca . The results of Real-time PCR analysis suggested that TaLhca expressed in root, stem and leaves , with a highest level in leaves, while a lowest level in root. Interestingly, the expression of TaLhca could be strongly induced by NaCl、drought、ABA and H2O2 and low temperature stress, while down regulated by darkness treatment. This results provided references for better understanding on the mechanism of photosynthesis and gene expression in wheat.