【Objective】 WRKY transcription factors are involved in regulation of low phosphorus stress in plants. Based on previous transcriptome data of Tartary buckwheat (Fagopyrum tataricum) under low phosphorus stress, the aim of this study is to isolate FtWRKY28 gene, to predict the structure of the gene and its deduced protein, to analyze the subcellular localization and transcription activating activity of the protein, and to investigate the gene expression patterns in different organs and under low phosphorus stress and hormone application, thus providing a basis for the function identification of the gene. 【Methods】 Specific primer sequences were designed according to the annotated Tartary buckwheat genome database. Reverse transcription PCR was used to amplify the entire coding sequence (CDS) of FtWRKY28 from the root RNA pools generated from Tartary buckwheat stressed by low phosphorus. Bioinformatical tools were employed to analyze the structures of the gene and protein and the phylogenetic relationships of homologous proteins. Real-time fluorescence-based quantitative PCR (qRT-PCR) was used to investigate the gene expression patterns. Transient expression system of Arabidopsis proplasts was used to analyze the subcellular localization of the protein. Yeast one-hybrid was employed to analyze the transcription activating activity of the protein. 【Results】 The obtained CDS of FtWRKY28 was 876 bp in length, encoding a polypeptide of 291 amino acid residues consisting of one conserved WRKY domain with a zinc finger motif of C2H2, thus belonging to the WRKY group II. FtWRKY28 was localized in nucleus, and had transcription-activating activity. The transcript abundance of FtWRKY28 was relatively higher in roots, and was significantly induced by low phosphorus and hormones such as indole acetic acid, gibberellin 3, and 6-benzylamino purine in roots. 【Conclusion】 Taken together, FtWRKY28 possesses basic structural and biochemical characteristics as a putative transcription factor, and may be involved in low phosphorus response possibly by crosstalk of auxin, gibberellin and cytokinin signaling networks.