Abstract:Abstract 【Objective】 Through the joint identification and analysis of disease resistance in the field and indoor seedling stage, the distribution of disease resistance of gossypium barbadense germplasm resources was mastered, which provided a basis for screening high resistance resources to Verticillium wilt. 【Methods】 The 238 gossypium barbadense germplasm resources of our research group were used as identification objects. In 2023, the natural disease field plot test was carried out in KEL and AWT, and the resistance of gossypium barbadense resources to Verticillium wilt was identified and evaluated by indoor inoculation. 【Results】 The genetic diversity of 15 indexes of 238 gossypium barbadense germplasm resources was analyzed. The coefficient of variation ranged from 2. 00 % to 36. 97 %, with an average of 16. 05 %. Resistance identification in different environments showed that the coefficient of variation ranged from 8.40 % to 71.06 %. Based on the analysis of four different environments, no germplasm resources with complete immunity and high resistance to Verticillium wilt were identified, and 30 resistant materials, 194 resistant materials and 14 susceptible materials were identified. 【Conclusion】 The overall trend can be seen that most of the gossypium barbadense planting resources have resistance to Verticillium wilt. Therefore, it is very important to identify and select germplasm resources with strong resistance to Verticillium wilt from gossypium barbadense germplasm resources.