积雪草主要三萜类化合物测定方法的优化研究
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1.西安文理学院;2.西安文理学院 美术与设计学院;3.西安交通大学药学院

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陕西省重点研发计划项目(2019SF-277)


#$NPOptimization of determination methods for major triterpenoid compounds in Centella asiatica (L.) Urban
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    摘要:

    【目的】建立以环糊精为流动相添加剂的高效液相色谱法测定积雪草药材中三萜类化合物积雪草苷、羟基积雪草苷和积雪草苷B的含量,为积雪草的质量控制提供依据。【方法】采用分子设计方法优选流动相添加剂-环糊精的种类,效应面法分析环糊精浓度、流动相中乙腈的比例、流动相pH对分离的影响,优化色谱条件,测定积雪草中积雪草苷、羟基积雪草苷和积雪草苷 B的含量。【结果】以γ-环糊精作为流动相添加剂,固定相为CAPCELL PAK C18柱(150 mm×4.6 mm,5 μm),流动相为乙腈-3 mmol/L环糊精溶液(含0.10%磷酸)为20∶80(V/V),流速为1.0 mL/min,柱温为25℃,检测波长为205 nm;羟基积雪草苷和积雪草苷 B分离度为4.32;积雪草苷在200~2 000 ng、积雪草苷B在120~1 200 ng、羟基积雪草苷200~2000 ng范围内线性关系良好,方法精密度、重现性、稳定性和回收率良好。【结论】该方法简便,准确可靠,且经济环保,可作为积雪草药材中主要三萜类化合物的测定方法。

    Abstract:

    [Objective] To provide a basis for quality control of Centella asiatica (L.) Urban, a high-performance liquid chromatography method using cyclodextrin as a mobile phase additive for the determination of asiaticoside, asiaticoside B and madecassoside was established. [Methods] Molecular design was used to optimize the type of the mobile phase additive-cyclodextrin. The response surface method was used to analyze the effects of cyclodextrin concentration, the proportion of acetonitrile in the mobile phase, and pH of the mobile phase on separation, and optimize the chromatographic condition. The optimized chromatographic condition was used to determine the content of asiaticoside, asiaticoside B and madecassoside in C. asiatica. [Results] γ- cyclodextrin was used as a mobile phase additive. The separation of asiaticoside, asiaticoside B and madecassoside was carried out on CAPCELL PAK C18 (150 mm×4.6 mm, 5 μ m) with 3 mmol/L γ-cyclodextrin solution (contained 0.10% phosphoric acid)-acetonitrile (20∶80, V/V). The detecting wavelength was 205 nm. The flow rate was set at 1.0 mL/min and the column temperature was 25 ℃. The resolution of asiaticoside B and madecassoside is 4.32. Linearity was good in the range of 200~2 000 ng for asiaticoside, 120-1 200 ng for asiaticoside B and 200~1 200 ng for madecassoside. The precision, reproducibility, stability, and recovery rate of method were good. [Conclusion] This method is simple, accurate, reliable, and environmentally friendly, and can be used as a quality control of major triterpenoid compounds in Centella asiatica (L.) Urban.

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杨若朦,田军,李蕴哲.积雪草主要三萜类化合物测定方法的优化研究[J].西北植物学报,2024,44(10):1639-1645

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  • 收稿日期:2024-05-06
  • 最后修改日期:2024-06-01
  • 录用日期:2024-06-04
  • 在线发布日期: 2024-08-20
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