旱柳失水枝条中ABA介导的代谢组响应分析
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中国林业科学研究院林业研究所

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国家自然科学(32071729)


Analysis of ABA-mediated metabolome response in dehydratedbranches of Salix matsudana
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    摘要:

    ABA是植物干旱胁迫的关键激素,通过代谢组学分析,揭示旱柳离体枝条ABA介导的失水响应差异代谢物及其相关通路,为旱柳干旱胁迫响应提供研究基础和理论依据。【方法】以旱柳离体枝条为材料,探究ABA合成抑制剂钨酸钠溶液浸泡后旱柳失水至不同水势枝条木质部代谢组的差异响应。【结果】失水至P50(-1.9 MPa)旱柳枝条代谢组与未失水枝条(0 MPa)相比,脱落酸含量显著增加了4.25倍,D-果糖等可溶性糖含量与组氨酸等氨基酸含量显著增加。ABA抑制剂处理后脱落酸含量显著降低了3.82倍,D-1,5-脱水果糖等可溶性糖含量与苯丙氨酸等氨基酸含量显著降低。植物激素信号转导、类胡萝卜素生物合成和淀粉与蔗糖代谢途径等是旱柳枝条失水响应的关键代谢通路。【结论】旱柳枝条失水后ABA含量显著增加,介导淀粉水解过程等降低了细胞渗透势以应对干旱胁迫。

    Abstract:

    【Objective】 ABA is a key hormone for drought stress in plants. Differential metabolites and their associated pathways of ABA-mediated dehydrated response in the isolated branches of Salix matsudana were revealed by metabolomics analysis, which provided the research basis and the theoretical basis for the response to drought stress in S. matsudana. 【Methods】The authors investigated the differential response of the xylem metabolomes of the S. matsudana branches dehydrated to the different water potentials after soaked in the sodium tungstate solution, an inhibitor of ABA synthesis, using the isolated branches of S. matsudana as materials. 【Results】 Compared with the deionized water-soaked non-dehydrated branches, the dehydrated to P50 water potential branches: The abscisic acid content was significantly increased by 4.25 times, and the content of the soluble sugars such as D-fructose was significantly increased along with the content of amino acids such as histidine. ABA synthesis inhibitor-soaked branches compared to deionized water-soaked branches: The content of abscisic acid was significantly reduced by 3.82 times, and the content of the soluble sugars such as D-1,5-defructose and amino acids such as phenylalanine was significantly reduced after soaked in the inhibitor of ABA synthesis. Plant hormone signal transduction, carotenoid biosynthesis and starch and sucrose metabolism pathways were the key pathways in the dehydrated response of S. matsudana branches. 【Conclusion】 In the S. matsudana branches after dehydration, the ABA content increased significantly, which mediated the starch hydrolysis process and other processes to reduce the osmotic potential of cells in response to the drought stress.

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胡耀芳,李奥,殷佳慧,等.旱柳失水枝条中ABA介导的代谢组响应分析[J].西北植物学报,2024,44(11):1735-1742

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  • 收稿日期:2024-05-26
  • 最后修改日期:2024-06-28
  • 录用日期:2024-06-28
  • 在线发布日期: 2024-09-25
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