丝瓜TIFY基因克隆及其组织表达分析
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金陵科技学院 园艺园林学院

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南京市品种选育专项项目(202210020);国家级大学生创新训练计划(202313573033Z);金陵科技学院高层次人才科研启动项目(jit-b-202009);金陵科技学院科研基金孵化项目(jit-fhxm-202113)


Cloning and Tissue Expression Analysis of TIFY genes in Luffa cylindrica
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    【目的】 TIFY基因在植物生长发育过程中发挥重要作用,本研究对丝瓜TIFY基因家族成员进行鉴定,筛选果实中高表达的成员,并对其进行克隆和组织表达分析,为阐明其生物学功能研究奠定基础。【方法】通过生物信息学方法对丝瓜TIFY基因家族成员进行鉴定,基于转录组分析筛选果实中高表达的成员,基于RT-PCR技术对其进行克隆,基于qRT-PCR研究其组织表达。【结果】丝瓜基因组中包含17个TIFY基因,不均匀地分布于9条染色体上,CDS序列全长297~1353 bp,可分为4个亚家族;LcTIFY2、LcTIFY7、LcTIFY11和LcTIFY13在丝瓜果实中的表达量显著高于其他成员;克隆了其CDS序列,分别具有3,5,6,2个外显子;上述4个基因均在丝瓜果皮中的表达量最高,此外,LcTIFY7和LcTIFY13在生殖器官中的表达量显著高于营养器官;LcTIFY11在雌花蕾和子房中的表达量显著高于雄花蕾。【结论】丝瓜TIFY家族成员LcTIFY2、LcTIFY7、LcTIFY11和LcTIFY13可能在其生殖生长和果实发育过程中发挥作用。

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    【Objective】TIFY transcription factors are involved in the regulation of growth and development processes in plants. This experiment involves the identification of the members of the TIFY family in Luffa cylindrica, selecting the highly expressed members in the fruit, and further cloning and investigating their expression patterns in different tissues, thus providing a basis for the functional identification of these genes. 【Methods】Bioinformatics methods were used to identify members of the TIFY gene family in L. cylindrica. Transcriptome analysis was performed to screen for highly expressed members in L. cylindrica fruit, followed by cloning using RT-PCR. Tissue expression was analyzed using qRT-PCR. 【Results】 The L. cylindrica genome contains 17 TIFY genes, unevenly distributed across 9 chromosomes, with CDS lengths ranging from 297 bp to 1 353 bp, classified into four subfamilies. Transcriptome analysis revealed that LcTIFY2, LcTIFY7, LcTIFY11, and LcTIFY13 are significantly more highly expressed in L. cylindrica fruit compared to other members. The CDS sequences of these genes were cloned, with structures comprising 3, 5, 6, and 2 exons, respectively. Tissue expression analysis indicated that all four genes have the highest expression in the peel of L. cylindrica fruit. Additionally, LcTIFY7 and LcTIFY13 showed significantly higher expression in reproductive organs than in vegetative organs, while LcTIFY11 exhibited significantly higher expression in female flower buds and ovaries than in male flower buds. 【Conclusion】The TIFY family members LcTIFY2, LcTIFY7, LcTIFY11 and LcTIFY13 may play roles in reproductive growth and fruit development of L. cylindrica.

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刘同金,刘振洋,班秋妍,等.丝瓜TIFY基因克隆及其组织表达分析[J].西北植物学报,2024,44(11):1752-1759

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  • 收稿日期:2024-06-05
  • 最后修改日期:2024-06-30
  • 录用日期:2024-07-01
  • 在线发布日期: 2024-09-24
  • 出版日期: 2024-11-04
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