Abstract [Objective] This study was designed to explore the tolerance strategies of different organs of ramie plants (Boehmeria nivea) to cadmium (Cd) stress. [Methods] Using the Cd-enriched germplasm "Xiangzhu XB" and the Cd-tolerant germplasm "Xiangzhu 3" as materials, a pot trial was conducted using that subjected to varying concentrations of Cd stress (0, 50, 100, 200 mg/kg). The subcellular distribution and chemical forms of Cd in different organs and the changes in the composition of underground organ cell walls were analyzed and compared. [Results] (1) To tolerant the Cd stress, ramie plant reduces the Cd translocation by adsorbing and immobilizing it in the cell walls and compartmentalizing within vacuoles. For example, over 90% of Cd2+ in the underground organs and leaves is immobilized through these mechanisms. (2) In the process of adapting to Cd stress, the Cd binding forms in different organs show a trend of shifting from highly mobile forms to less mobile ones. Under Cd stress, Cd in the underground organs predominantly exists in a NaCl-extractable form, accounting for more than 50% of the total Cd content. (3) Factors influencing Cd retention in underground organs include an increase in the cell wall components and the proportion of NaCl-extractable Cd in these organs. Additionally, the retention effect is influenced by the genotype. [Conclusion] The organs of ramie enhance Cd tolerance by altering the binding forms of Cd. The cell wall and vacuoles play important roles in improving Cd tolerance. The increase in major components of the cell wall in the underground organs contributes to Cd retention.