【Objective】 Suberin is a polyester-type biopolymer that specifically deposits in tissues such as the endodermis, periderm, seed coat, and wound epidermis of plants. Acting as a barrier between the plants and their environment, suberin controls the transport of water and solutes, protecting plants from environmental stresses and pathogenic invasion. This article reviews the key enzymes and transcription factors involved in suberin biosynthesis, discusses the impact of environmental factors on suberin synthesis and deposition, and looks ahead to future research directions.【Reviews】 The regulation of suberin involves various factors, including key enzymes such as β-ketoacyl-CoA synthase (KCS), fatty acyl reductase (FAR), cytochrome P450 enzymes (CYP family), and transcription factors such as MYB, NAC, WRKY, etc. Environmental factors also regulate the biosynthesis and deposition of suberin through complex signal transduction pathways. 【Prospect】 Future research should focus on the key regulatory nodes of suberin biosynthesis, particularly exploring the factors that influence species-specific deposition of suberin in crops. By leveraging multi-omics approaches to elucidate its transport, assembly, and post-transcriptional regulation mechanisms, there is potential to provide new strategies for enhancing nutrient use efficiency and stress resistance in crop genetic improvement.