Anatomical and histochemical methods were used to investigate micromorphological characters and locality of catalpol and polysaccharide in Rehmannia glutinosa leaves.(1)Both adaxial and abaxial leaf surface of R.glutinosa possess glandular and non-glandular trichomes.Glandular trichomes can be classified into short-stalked and long-stalked.The secretion of these glandular trichomes is mainly compounds of flavnoids and polysaccharide.Anomocytic stomata are distributed on both leaf surfaces.Although stomatal density of abaxial surface is well above than that of adaxial surface,but stomatal index difference is modest.Palisade parenchyma cells are arranged densely in two to three layers.Irregular spongy parenchyma cells have irregular-shaped cells with large intercellular spaces.(2)Histochemical tests indicated that yellow-speckled spongy parenchyma cells which concentrate at the end of the teeth near the leaf margin contain a combination of catalpol and polysaccharide.Parenchyma cells in cortex,phloem and xylem are locality of catalpol and polysaccharide.