Based on the cDNA sequence of msLTP related to male sterility of Chinese cabbage-pak-choi (Brassica campestris ssp.chinensis var.communis,syn.B.rapa ssp.chinensis var.communis),a pair of specific primers were designed and a 312 bp fragment was amplified from the cDNA of flower buds of Chinese cabbage-pak-choi.The fragment was introduced to binary vector pBI121,and the generated antisene RNA vector was then mobilized into Agrobacterium tumefaciens strain LBA4404.The A.tumefaciens harboring the msLTP fragment was transformed to flowering Chinese cabbage (B.campestris ssp.chinensis var.parachinensis) via tissue culture and Agrobacteriummediated transformation.The transgenic plants were confirmed by PCR and Southern blot,and 25 independent transgenic plants were obtained.The pollen grains from transgenic plants exhibited abnormal shapes,and only 38.56% of pollen grains from these plants germinated normally.The germination rate was reduced 37.76 percentage points than that of pollen grains from nontransgenic plants (76.32%).These results indicated that msLTP gene interrupted by antisense RNA resulted in partial pollen grains abortion in flowering Chinese cabbage,suggesting that msLTP gene possesses an important role in pollen development of Chinese cabbage crops such as Chinese cabbage-pak-choi and flowering Chinese cabbage.