The effects of the plant cytoskeleton labels,GFP-FABD2 and GFP-MBD were investigated on Arabidopsis callus induction,suspension cells cultivation and capacity of response to stress.The results showed that the expression of these two fusion proteins diversely effected the callus induction and suspension cell growth.(1)GFP-MBD expression extended the time of callus induction,altered the callus morphology,reduced the quantity of callus to 59% of wild type,decreased the major/minor axial ratio of suspension cells to 1.20±0.21,reduced the relative viability of 7-day-old suspension cultures to 0.66±0.09 and altered cell growth curve.(2)GFP-FABD2 expression had no apparent effects on callus,but it significantly increased the major/minor axial ratio of suspension cells to 2.49±1.18,reduced the relative viability of 7-day-old suspension cultures to 0.87±0.06 and effected cell growth curve.(3)These effects can be restored by adjusting the hormone level.(4)In the optimized condition,we did not score any significant deviation in temperature,osmotic pressure,mechanical stress and other stress from those found in wild type Arabidopsis suspension cells.