Abstract:In this paper,we used cDNA-AFLP to isolate differentially expressed genes in sugarcane induced by GA3.Sugarcane plants (Saccharum officinarum cv.ROC22) which were sprayed with the concentration of 200 mg·L-1 gibberellin at early elongation stage as treatment,while sprayed with distilled water as control.(1)One hundred and eighty six bands of differential expression genes were obtained by 169 pairs of selective amplification primers.After reverse Northern blot analysis,56 positive TDFs were selected for sequencing.(3)BLAST analysis showed the genes related to energy and metabolism (6),unknown functional protein (17),plant resistance (1),cell wall biosynthesis and modification (1),signal transduction (2),transcription factor (1) and others (28).Our result provides reference for investigating the molecular mechanism of sugarcane stem elongation.
