In this study,the male sterile wolfberry (Lycium barbarum Linn.)‘Ningqi 5’and normal fertile line ‘Ningqi 1’ were used.The total RNA was extracted from different developmental stages of bud,and reversely transcribed to the first strand of cDNA.In the light of β-1,3-glucanase homologous characteristic,callase gene （LG1） was cloned,and semi-quantitative RT-PCR analysis are carried out later.The results indicated that:(1)LG1 belongs to plant glycosyl hydrolase family 17 genes,and encode 344 amino acids.Five conserved regions of amino acids exist in four plant anther β-1,3-glucanase genes.(2)LG1 gene were highly expressed in the normal fertile wolfberry,but silenced in the male sterile wolfberry.Its nucleotide sequence even has no any mutation in the sterile wolfberry.(3)The anther were stained with aniline blue secondly.It showed that the normal fertile wolfberry anthers tetrad callose was completely disintegrated,while the male sterile line’s was blocked.Therefore,LG1 gene is a downstream gene regulated by upstream sterile gene in the pollen abortive process,its silencing is an important reason for wolfberry sterility.