In order to further understand the mechanism of adventitious root formation and development of Tetraena mongolica Maxim.,we studied the dynamical changes of starch grain and protein accumulation by PAS (Periodic acid-Schiff)/S (Naphthol yellow S) in the origin and development of adventitious roots of T.mongolica Maxim..Results after cutting indicated that:（1）Starch grains were accumulation in the parenchyma cells between phellogen cambium and vascular cambium in stem.Starch grains achieved height value was reduced gradually with callus development continued in the callus development primary.(2)Proteins were reduced in the parenchyma cells between phellogen cambium and vascular cambium in stem.Parenchyma cells contains a little of proteins after callus formed.(3)Starch grains and proteins were mainly distributed in vigorous dividing cells,organ primodium,and adventitious roots cells and nearby cells.Protein and starch provided material and energy to formation and development of adventitious roots of T.mongolica Maxim..