The better understanding of lily pollen storage characters not only provides scientific basis for solving different flower seasons and long distance hybridization during traditional crossing breeding,but also is essential for the storage of lily germplasm resources.Thus,pollens of twelve cultivars belonging to the Asiatic,Oriental and OT hybrids were used as research materials to find out the best media formula for most cultivar’s pollen.The time to survey the germination rate of lily pollen,different flower bud development phase ［color fully developed (-1 level),2/3 color developed (-2 level)] and treatments after collecting pollen ［tubes (No.1),putting cotton in tubes (No.2),putting cotton and drier in tubes (No.3)] were determined and discussed in this research.The results showed that:(1)Liquid culture medium containing 5% sucrose,formula 100(50) mg/L boric acid and 30(50) mg/L CaCl₂ was optimal for most cultivars.It was most suitable that testing the pollen germination rate after 24 h culturing.(2)The germination rate of pollens in -1 level was higher than those in -2 level.The -1 level’s pollen germination rate of ‘Tresor’ can reach 83.2% while -2 level was just 21%.In room temperature,the time of pollens keeping their vitality was the shortest.Most cultivar’s pollen couldn’t germinate after 20 days storage.4 and -20 ℃ were more suitable for short-time storage of pollen with no significant difference between them.(3)The Asiatic lily cultivars maintain 24.7%～55.9% germination rate while the Oriental lily cultivars maintain 6.8%～12.7% after 120 days storage.In -80 ℃,pollens maintain higher germination rates which were 26.7%～61.9% for the Asiatic lily cultivars and 7.1%～16.2% for the Oriental lily cultivars.(4)In three treatment methods,the germination rate of pollens in tubes with cotton and drier which was No.3 was higher than others under different storage temperatures.In summary,the best storage condition for lily pollen was putting cotton and drier in tubes (No.3) at -80 ℃.