Using Yunnan potato varieties including ‘Jianchuanhong’ mother plant and its 70 inbred seedlings from one berry,and 100 seedlings of B20 hybrid [CIP010(♀)×CIP004(♂)] as materials,we studied their genetic diversity with 12 pairs of SSR primers,for the purpose of finding the consistent progenies as mather plant (parent) at the molecular level and providing the basis for long-term preservation of germplasm resources.The results showed that:(1)The polymorphism rate of ‘Jianchuanhong’ seedlings population was 81.6%,which was higher than that of B20 with 72.8%,which indicated that the polymorphism rate of two groups were higher.(2)The cluster analysis indicated that the offsprings of both inbred or hybrid populations had high genetic similarity coefficient,the range from 0.74 to 0.96.Two groups had different degrees of genetic segregation which were small,most of amplified bands were consistent.(3)We did not found one plant from a berry which was as same as ‘Jianchuanhong’ mother plant at the molecular level in all the tested materials.So it was very difficult to find the seedling populations which was not separated completely.We must be further evaluate the field performance of similar lines compared with mother plant (parent) at the molecular level,and determine whether they can recover the sources by similar or poles near lines.