In this study,a kind of psammophytes,Caragana korshinskii seeds,were used as experiment material.The seeds were treated by electric field and the seedling growth was under drought stress conditions abd the expression difference of genes in the C.korshinskii young leaves were tested by mRNA differential display technology(DDRT-PCR).In order to compare the gene expression difference,young leaf tissues were classified three samples include nature control(called CK),drought stress control(called SCK) and electric field treatment group(strength 2 kV/cm,frequency 15 kHz,time 5 min,called ET).The 3 anchor primers and 26 random primers were used to form 78 primer pairs.The DDRT-PCR was carried out 234 PCR reactions for the three kinds of samples.The cDNA bands showed significant difference between three young leaf tissue samples.(1)Compared with CK,SCK appeared 282 novel bands and disappeared 179 bands,and ET appeared 303 novel bands and disappeared 200 bands.Besides,compared with SCK,ET appeared 236 bands and disappeared 257 bands.(2)The novel bands were recovered and three differentially expressed cDNA fragments were obtained.All of the expressed sequence tags(EST) had the homology with known cDNA clones in Genbank databases.EST-1 was similar to Glycine max uncharacterized LOC100305895(LOC100305895) and the identity was 76%;EST-2 was similar to Cicer arietinum probable UDP-N-acetylglucosamine——peptide N-acetylglucosaminyltransferase SEC-like(LOC101506182),transcript variant X2 and the identity was 88%;EST-3 was similar to Lotus japonicus clone JCVI-FLLj-5M12 and the identity was 88%.(3)After Blastx comparision,EST-1 was similar to the 145-195 aa of 1-deoxy-D-xylulose 5-phosphate reductoisomerase(390 aa) and the identity was 52%;EST-2 was similar to the 706-744 aa of O-linked GlcNac transferase like protein(744 aa) and the identity was 97%;EST-3 was similar to acyl-CoA-binding protein(90 aa) and the identity was 97%.Our result provides experimental basis to analyze the biological mechanisms of drought resistance improvement induced by electric field treatment on plant seeds from the gene expression difference.