The seedling growth,cell protective enzyme activities,soluble sugar and candied acid contents and conductivity changes of ‘Yichengqingpidazi’ seeds treated with pre-chilling(2-4 ℃) for different days(5,10 and 15 d) under different salinity gradient(0,0.1%,0.2%,0.3%,0.4%,0.5% and 0.6%) were studied in order to clarify the effects of pre-chilling Punica granatum on seed germination and seedling physiological and biochemical characteristics.The results showed that:(1)Low temperature pretreatment and low salinity stress can increase the pomegranate seed germination rate and germination potential,low temperature processing 5 d and 0.1% salt environment conducive to the growth of seedlings.(2)Low temperature processing(5 d) can significantly increase the protective enzyme(SOD,POD,CAT) activities of pomegranate seedlings,prolonged cold treatment(15 d) reduced the protective enzyme activities of seedlings;Pomegranate seedlings were occurred in salt damage when the salt concentration is higher than the 0.3 percent beyond the protective enzyme system adjustment range.(3)Seedlings decreased contents of soluble protein and proline under 5 d and 10 d pre-chilling.With salt concentration increasing,soluble protein and proline contents were gradually increased.(4)5 d and 10 d pre-chilling can improve the pomegranate seedling membrane permeability,relative conductivity also increased significantly with increasing salt concentration.The study indicated that in low-salt environment 2-4 ℃ pre-chilling Pomegranate seedling could adjust growth enhance seedling osmotic adjustment ability to ensure normal growth of the plants needed water,while increasing the contents of soluble protein and proline to ensure their own protection seedling synthase,maintain normal metabolic activity of cells.In addition,by reducing the cell membrane lipid peroxidation to ensure the integrity of the cell membrane to improve its ability to adapt to adversity,thus effectively preventing membrane lipid peroxidation damage to plants,it showed strong salt resistance.