Abstract:In order to study the molecular adaptation mechanism of the alpine plant Saussia medusa to high ultraviolet radiation, we cloned the cDNA and promoter sequences of anthocyaninrelated SmMYB1 gene by RTPCR combined with RACE methods and hiTAIL PCR. The results show that: (1) the length of SmMYB1 cDNA (GenBank: MT188353) is 1 011 bp encoding 269 amino acids, and the sequence of gDNA contains two introns and three extrons. (2) Bioinformatics analysis show SmMYB1 protein has more high similarity with MYB in Asteraceae contained the [DE]Lx(2)[RK] x(3)Lx(6)Lx(3)R and ANDV motifs, and belongs to the sixth subfamily of Arabidopsis thaliana. The length of SmMYB1 promoter (GenBank: MT188354) is 1 407 bp, which contains many light responsive elements. (3) qRTPCR shows that SmMYB1 gene is expressed in roots, stems, leaves and flowers, and the highest expression emerges in the flowers. Under ultraviolet radiation stress, the expression of SmMYB1 reaches the highest at 4 h, and then decreases gradually. It is speculated that SmMYB1 can regulate anthocyanin synthesis and is involved in the UV responsive pathway in S. medusa.