HAM subfamily genes of the GRAS family are important regulators for maintaining the undifferentiated state of shoot apical meristem (SAM), and affect the process of floral transition process in plants. In this study, based on the homologous sequences of HAM subfamily genes from a transcriptome dataset, three HAMlike genes were cloned from Chrysanthemum lavandulifolium by RTPCR technology. Sequence analysis showed that the open reading frames (ORF) of the three genes were 1 845, 1 479 and 1 881 bp in length, encoding 614, 492 and 626 amino acids, respectively. Blastp analysis showed that the coding products of the three genes contain typical structures of HAM subfamily, and show high identity with the SCL6 protein of Artemisia annua, which reach up to 94.39%, 91.90%, and 94.27%, respectively. Further analysis showed that amino acid sequences translated by the three genes had the closest evolutionary relationship to SCL6 in all Arabidopsis GRAS family members, so they were named as ClSCL6a, ClSCL6b, ClSCL6c, respectively. Fluorescence quantitative analysis showed that the three genes were highest expressed in the stems of C. lavandulifolium, and generally with low expression level in roots and flowers. In floral organs of different development stages, all the three ClSCL6 genes were expressed. ClSCL6a and ClSCL6c reached their expression peak before the pollinating period, while ClSCL6b had the highest expression level in the period of small flower buds. This study laid the foundation for further studying on the role of ClSCL6 in floral transformation of C. lavandulifolium.