Abstract:In this study, we screened the members of the MYB transcription factor family of T. vernicifluum. After homology comparison, open reading frame (ORF), basic sequence characteristics, etc., TvMYB1 was selected for basic biological information and stress expression analysis. Toxicodendron vernicifluum potted seedlings were used as materials, RTPCR technology was used to clone the cDNA of the TvMYB1 gene of the lacquer tree, and the TvMYB1 gene of the lacquer tree was cloned using the cDNA of the stem and root of the lacquer tree as a template, and the realtime fluorescence quantitative (qRTPCR) analysis was used. After PEG6000 stress, its expression changes in roots, stems and leaves with the change of treatment time. The results showed: (1) bioinformatics analysis showed that the ORF of the TvMYB1 gene is 900 bp long, the encoded protein contains 299 amino acids, the molecular mass is 32.45 kD, and the theoretical isoelectric point is 9.51. The protein structure contains 1 incomplete MYB repeat, the subdomain belongs to the 1RMYB class, and is subcellularly located in the nucleus. (2) Phylogenetic tree analysis showed that the protein encoded by the TvMYB1 gene is closely related to the MYB protein of pistachio (Pistacia vera), cocoa (Theobroma cacao), and citrus (Citrus sinensis). (3) The results of realtime fluorescent quantitative PCR (qRTPCR) showed that after PEG6000 treatment, TvMYB1 can be induced to express in different tissues of T. vernicifluum, with the highest expression in leaves, but relatively lower expression in roots and stems. With the extension of the treatment time, the expression of TvMYB1 in roots and stems has an increasing trend, but the opposite is true in leaves. Therefore, TvMYB1 is induced by osmotic stress, plays a role in T. vernicifluum stress response, and is an important candidate gene for research on the stress adaptation mechanism of T. vernicifluum.