Stearoylacyl carrier protein desaturase (SAD) catalyzes the dehydrogenation of stearic acid to oleic acid, which is the key enzyme to form unsaturated fatty acids. The Perilla frutescens stearoylacyl carrier protein dehydrogenase (PfSAD) family genes were screened and identified from the transcriptome database of perilla, and bioinformatics analysis and conserved functional domain analysis were carried out. The expression characteristic of each member of PfSADs in different tissues were detected by qRTPCR. So as to explore the role of PfSAD family genes in regulating seed fatty acid components, and provide gene elements for the genetic improvement of fatty acid components of P. frutescens. The results showed that: (1) six PfSAD family genes were detected from P. frutescens transcriptome database tested by our research group in the early stage, and the amino acid lengths of the encoded proteins were between 367 and 396 amino acids. They all had the conserved domain and diiron center of SAD, and the encoded proteins were all located in the chloroplast. (2) The results of multiple sequence alignment showed that the protein sequences of PfSADs had more than 50% homology with the SAD protein sequence of Arabidopsis thaliana, Ricinus communis and Theobroma cacao L.; Phylogenetic tree analysis showed that six PfSADs proteins belonged to three subgroups, respectively, among which the first subgroup contained PfSAD1, the second subgroup contained PfSAD2 and PfSAD3, and the third subgroup contained PfSAD4, PfSAD5 and PfSAD6. (3) Real time quantitative PCR analysis showed that there were significant differences of the expression levels of PfSAD members in different tissues of ‘JinZisu 1’, in which PfSAD1 is mainly expressed in leaves, the expression levels of PfSAD2, PfSAD3, PfSAD4 and PfSASD5 were higher in seeds, and PfSAD6 has a significant expression advantage in flowers. The results show that PfSADs have typical conserved motifs and active center that catalyze SAD, and their members are highly expressed in different tissues, It suggested that all these 6 genes involved in the formation of oleoylACP derived from the dehydrogenation of stearoylACP and play a key role in the process of P. frutescens oil synthesis and metabolism. The study provides an important reference for further exploring the role of PfSAD family genes in regulating seed fatty acid components.