To understand the biological function and expression patterns of the longan WOX (DlWOX) family, we identified the members of DlWOX family, analyzed bioinformatics based on the longan genomewide database. With quantitative realtime PCR (qRTPCR) technology we detected and verified their expression patterns in the three early stages of somatic embryogenesis in longan and under different hormone treatments. The results showed that: (1) 13 longan DlWOX family members were screened, all of which are unstable proteins. The subcellular localization prediction results showed that DlWOX family members are localized on the nucleus and cytoskeleton. Phylogenetic tree analysis found that the DlWOX family is divided into ancient branches, middle branches and WUS (The WUSCHEL gene is the first gene discovered in the WOX family) branches. (2) Gene structure analysis revealed that DlWOX family members contained 0-19 number of introns, and most of their encoded proteins contained motif1 and motif2, and some members contain specific motifs. The cisacting elements of the DlWOX promoter contained a large number of light and hormone response elements. (3) Analysis of the expression patterns of DlWOX in different tissues of longan and early somatic embryogenesis revealed that some members of this family are highly expressed in the leaves, and DlWOX14.1, DlWOX14.2 and DlWOX9A were highly expressed in the embryogenic callus (EC) stage. QRTPCR analysis of DlWOX under different hormone treatments of longan EC showed that most of the DlWOX family members responded to the methyl jasmonate (MeJA) and gibberellin (GA) hormones. Except for DlWOX6, the remaining members were upregulated under the GA and MeJA treatments. Among them, the expression of DlWOX9A was significantly upregulated under the treatment of GA and MeJA. The study found that the expression levels of the longan DlWOX9A based on the transcriptome sequencing results and the qRTPCR results were different and the trends were not exactly the same. It was speculated that the WOX family plays an important role during the early somatic embryogenesis of longan, especially in the GE stage. The DlWOX genes were highly conserved during evolution. Some members of the DlWOX family may play a role during the somatic embryogenesis of longan by responding to GA and MeJA hormones.